摘要
目的探讨肿瘤坏死因子(TNF)基因rs1800629、rs361525位点多态性及基因—环境交互作用与不明原因复发性流产(URSA)易感性的关系。方法使用SNaPshot多重SNP分型技术对150例URSA患者和150名健康对照检测TNF基因相关位点多态性,采用关联分析和广义多因子降维法(GMDR)分析其与URSA的关系。结果多因素回归分析显示,在共显性、显性和隐性遗传模式以及等位基因模式下,TNF基因rs1800629、rs361525位点均未发现与URSA发病风险增加有关(P>0.05)。但按流产次数进行分层分析,结果显示rs361525位点在显性遗传模式中,A等位基因携带者显著增加发生2次流产的风险(OR=2.402,95%CI:1.041~5.545)。GMDR分析显示,rs1800629、rs361525,工作属性存在交互作用。结论rs361525位点A等位基因可能为中国兰州地区URSA患者,特别是流产2次患者的易感等位基因。TNF基因-环境交互作用可能增加URSA发病风险。未发现TNF基因rs1800629多态性与USRA之间的联系。
Objective To investigate loci polymorphisms of TNF gene rs1800629 and rs361525 and the association of gene-environment interaction with the susceptibility to unexpected recurrent spontaneous abortion(URSA).Methods Genotyping of TNF gene(rs1800629 and rs361525)was performed on the samples from 150URSA patients and 150 healthy volunteers using SNaPshot method.Correlation analysis and generalized multifactor dimensionality reduction(GMDR)were used to analyze the relationship between URSA and polymorphisms of two loci.Results Multiple regression analysis showed that no significant difference in genotype of the TNF gene rs1800629 and rs361525 was observed between the case group and the control group(P<0.05).However,after stratified analysis,we found that in the dominant inheritance mode of rs361525 locus,the risk of RSA in A allele carriers significantly was increased in twice(OR=2.402,95%CI:1.041~5.545).GMDR analysis showed a significant gene-environment interaction among rs1800629,rs361525 and job′s character.Conclusion The TNF gene A allele of rs361525 is associated with URSA(n=2)susceptibility and the TNF gene-environment interaction may increase the risk of USRA.No association between rs1800629 and USRA is found.
作者
李静
裴建赢
刘倩
王文第
金玉霞
毛宝宏
LI Jing;PEI Jianying;LIU Qian;WANG Wendi;JIN Yuxia;MAO Baohong(Scientific Research Center,Gansu Provincial Maternity and Child-Care Hospital,Lanzhou 730050,China;Laboratory Medicine Center,Lanzhou Uni-versity Second Hospital,Lanzhou 730020,China)
出处
《实用医学杂志》
CAS
北大核心
2022年第22期2822-2827,共6页
The Journal of Practical Medicine
基金
兰州市科技发展指导性计划项目(编号:2020-ZD-14)
甘肃省卫生行业科研计划项目(编号:GSWSKY2018-54)。
