摘要
将红细胞膜和4T1乳腺癌细胞膜形成的杂合细胞膜包覆在mPEG-PLGA搭载Verteporfin形成壳结构表面,合成新型的仿生纳米递送载体RT@VNPs。采用透射电镜、纳米粒度及Zeta电位分析仪、紫外–可见波谱仪、荧光波谱仪等检测仿生纳米载体的基础性能。在此基础上,以乳腺癌细胞4T1为研究对象,评估体外RT@VNPs的细胞摄取、ROS生成和杀伤能力;此外,通过测定免疫原性细胞死亡相关指标HMGB1和ATP,进一步分析RT@VNPs诱导肿瘤细胞产生免疫原性细胞死亡效应的能力。
The hybrid cell membrane formed by erythrocyte membrane and 4T1 breast cancer cell membrane was cloaked on the surface of mPEG-PLGA loaded with Verteporfin to form a shell structure,and a novel biomimetic nano-delivery carrier RT@VNPs was synthesized.The basic properties of biomimetic nanocarriers were analyzed by transmission electron microscopy,Zetasizer Nano ZS instrument,ultraviolet-visible spectrometers,and fluorescence spectrometers,etc.On this basis,4T1 cells were used to evaluate the cellular uptake,ROS generation,and killing abilities of RT@VNPs in vitro.In addition,the ability of RT@VNPs induced 4T1 cells to produce immunogenic cell death effects was further analyzed by measuring the immunogenic cell death-related indicators HMGB1 and ATP.
作者
焉绍辉
孙枝红
刘杰
孙成铭
YAN Shaohui;SUN Zhihong;LIU Jie;SUN Chengming(Binzhou Medical University,Yantai 264003,China;Yantai Yuhuangding Hospital Affiliated to Qingdao University,Yantai 264000,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2022年第8期1635-1643,共9页
Chinese Journal of Cell Biology
基金
烟台市科技计划项目(批准号:2022MSGY074)资助的课题。
