摘要
目的:分析不同杀青方式对地黄叶化学质量特征及抗氧化活性的影响,为地黄叶的加工工艺选择及质量评价奠定基础。方法:分别采用55℃杀青、沸水杀青与105℃杀青3种方法对地黄叶进行处理,杀青处理后样品55℃烘干。采集各处理样品的高效液相色谱法(HPLC)指纹图谱、近红外光谱(NIRS)指纹图谱,并利用2,2-联苯基-1-苦基肼基(DPPH)法分析其抗氧化活性。利用相似度分析、主成分分析(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)及Pearson相关性分析等方法对不同杀青处理地黄叶的质量进行综合评价。结果:HPLC、NIRS指纹图谱分析结果均显示,不同杀青处理的地黄叶质量特征存在差异,其中55℃杀青样品的化学质量综合得分最高,平均综合得分2.096分,105℃杀青次之,最低为沸水杀青。55℃杀青地黄叶的抗氧化活性最强,沸水杀青次之。OPLS-DA结果表明,引起3种杀青处理地黄叶化学质量产生差异的主要成分为毛蕊花糖苷、异类叶升麻苷和梓醇,这3种成分与地黄叶的抗氧化活性均呈正相关,其中毛蕊花糖苷、异类叶升麻苷与抗氧化活性的相关性达显著水平。结论:不同杀青方法对地黄叶的化学质量及抗氧化活性均有一定影响,其中低温杀青为地黄叶最佳的初加工方式。
Objective:To analyze the effect of different fixation methods on the chemical quality characteristics and antioxidant activity of Rehmannia glutinosa leaves,so as to lay a foundation for the selection of processing technology and quality evaluation of this medicinal materials.Method:R.glutinosa leaves was dried at 55 ℃ after treating by three fixation methods(55 ℃,boiling water,105 ℃),and then the fingerprints of R.glutinosa leaves were collected by high performance liquid chromatography(HPLC)and near infrared spectroscopy(NIRS),and their antioxidant activities were analyzed by the 2,2-biphenyl-1-picrylhydrazyl(DPPH)method.Finally,similarity analysis,principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA) and Pearson correlation analysis were used to comprehensively evaluate the quality of R.glutinosa leaves with different fixation methods.Result:The results of HPLC and NIRS fingerprint analysis indicated that there were differences in the quality characteristics of R.glutinosa leaves by different fixation treatments.The comprehensive score of chemical quality of R.glutinosa leaves by fixation at 55 ℃ was the highest,its average comprehensive score was 2.096,followed by fixation at 105 ℃,and the lowest was fixation with boiling water.The antioxidant activity of sample with fixation at 55 ℃ was the highest,followed by fixation with boiling water.The results of OPLS-DA showed that verbascoside,isoacteoside and catalpol were the main components causing the difference in chemical quality of the leaves from the three treatments,and the three components were positively correlated with the antioxidant activity of R.glutinosa leaves.Among them,the correlation between verbascoside and antioxidant activity was extremely significant,and the isoacteoside was significant.Conclusion:The chemical quality and antioxidant activity of R.glutinosa leaves are affected by the method of fixation,and the fixation at low temperature is the best primary processing method of R.glutinos
作者
崔锦鹏
耿晓桐
程梦娟
李伟峰
张娟
刘庆普
雷敬卫
谢彩侠
CUI Jinpeng;GENG Xiaotong;CHENG Mengjuan;LI Weifeng;ZHANG Juan;LIU Qingpu;LEI Jingwei;XIE Caixia(Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China;Xinyang Agriculture and Forestry University,Xinyang 464000,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2022年第23期197-204,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(82073952)。
关键词
地黄叶
杀青方法
指纹图谱
抗氧化活性
质量评价
高效液相色谱法(HPLC)
近红外光谱(NIRS)
Rehmannia glutinosa leaves
fixation method
fingerprint
antioxidant activity
quality evaluation
high performance liquid chromatography(HPLC)
near infrared spectroscopy(NIRS)
