摘要
[Objectives]This study was conducted to study the total polyphenol content and antioxidant capacity of different extraction fractions of Pholidota chinensis.[Methods]Different polar fractions of P.chinensis were obtained by ultrasonic extraction method,and determined for polyphenol contents by UV spectrophotometry,and their antioxidant activity was evaluated by DPPH free radical scavenging and ferric ion reducing antioxidant power(FRAP).SPSS analysis software was used to analyze the correlation between total polyphenol contents and antioxidant activity of different extracts.[Results]The contents of total polyphenols in different polar fractions ranged from 1.52 to 6.41 mg/g,and the order from low to high was:water<petroleum ether<ethyl acetate<95%ethanol.The reduction capacity of the total antioxidant capacity method ranged from 12.54 to 22.45μmol/L,and the order was 95%ethanol>ethyl acetate>petroleum ether>water.The DPPH median inhibition concentration ranged from 0.35 to 2.65 mg/ml,and the order was water>petroleum ether>ethyl acetate>95%ethanol.The total polyphenol content of P.chinensis was best in the 95%ethanol extraction fraction,which showed the strongest total antioxidant capacity,so the total polyphenol content of the 95%ethanol fraction was positively correlated with its antioxidant capacity,while the DPPH free radical-scavenging capacity was better in the water fraction,indicating that the free radical-scavenging capacity of the water fraction was better.[Conclusions]This study provides a theoretical basis for the further study of the chemical components of P.chinensis and the development and utilization of natural antioxidants.
基金
Supported by Guangxi Science and Technology Base and Talent Project(GK AD21238031)
Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine(GKJZ[2014]32)
Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine(GJKY[2013]20)
Guangxi Engineering Research Center of Ethnic Medicine Resources and Application(Guifa Gai High Technology Letter[2020]No.2605)
The Eighth Batch of Guangxi Specially-employed Expert Projects(GRCTZ[2019]13)
Youth Fund Project of Guangxi University of Chinese Medicine(2019QN012)
Guangxi Key Research and Development Project(GK AB21196016)
National Natural Science Foundation of China(82060695)
Guangxi Zhuang Autonomous Region Philosophy and Social Science Planning Research Project(20BMZ005)
Foreign Expert Project of the Ministry of Science and Technology of the People s Republic of China(GXL20200233001)
Guangxi First-class Discipline of Traditional Chinese Medicine:Ethnic Medicine(GJKY[2018]12).
