摘要
目的评价血府逐瘀汤对非酒精性脂肪性肝病(NAFLD)的药效,解析血府逐瘀汤发挥效应的物质基础。方法实验一探究血府逐瘀汤对高脂饮食诱导的小鼠NAFLD的作用,将50只C57BL/6J健康雄性小鼠随机分为正常组、模型组、血府逐瘀汤高剂量和低剂量组、奥贝胆酸对照组,每组10只。正常组小鼠予对照饮食,其余各组高脂饮食,造模第13周开始灌胃给药,16周末取材。记录进食量、体质量,酶联免疫法测定空腹血清胰岛素水平,检测空腹血糖并计算胰岛素抵抗指数。HE染色和NAFLD活动评分(NAS)观察小鼠肝组织病理;油红O染色观察脂质沉积;检测肝组织TG和血清ALT水平。实验二分析血府逐瘀汤各提取部位对高脂饮食诱导的小鼠NAFLD的作用,利用水煎、水提醇沉、石油醚萃取技术,获得血府逐瘀汤提取部位1、2、3。将54只C57BL/6J健康雄性小鼠随机分为正常组、模型组、血府逐瘀汤提取部位1、2、3和血府逐瘀汤对照组,每组9只。正常组予对照饮食,其余各组高脂饮食,造模第13周开始灌胃给药,16周末取材。记录进食量、体质量,酶联免疫法测定空腹血清胰岛素含量,检测空腹血糖并计算胰岛素抵抗指数。HE染色和NAS评分观察小鼠肝组织病理;油红O染色观察脂质沉积;检测肝组织TG、GGT、血清ALT水平。正态分布的计量资料两组间比较采用t检验;多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。非正态分布的计量资料多组间比较及进一步两两比较均采用Kruskal-Wallis H检验。结果实验一,血府逐瘀汤高、低剂量组和奥贝胆酸对照组均较模型组降低小鼠体质量和胰岛素抵抗指数(P值均<0.05),肝组织空泡状脂滴分布、小叶内炎症减少,肝细胞气球样变减轻,NAS评分下降(P值均<0.05),降低肝脏TG、血清ALT水平(P值均<0.05)。血府逐瘀汤高、低剂量降低体质量、胰岛素抵抗指数和NAS总分的作用
Objective To investigate the effect of Xuefu Zhuyu decoction on nonalcoholic fatty liver disease(NAFLD)and its material basis.Methods In experiment 1 for exploring the effect of Xuefu Zhuyu decoction on mice with NAFLD induced by high-fat diet,50 healthy male C57 BL/6 J mice were randomly divided into normal group,model group,high-and low-dose Xuefu Zhuyu decoction groups,and obeticholic acid control group,with 10 mice in each group.The mice in the normal group were given control diet,and those in the other groups were given high-fat diet.Gastric administration was started at week 13,and related samples were collected at the end of week 16.Food intake and body weight were recorded,enzyme-linked immunosorbent assay was used to measure the serum level of fasting insulin,fasting blood glucose was measured,and insulin resistance index was calculated.HE staining and NAFLD activity score(NAS)were used to observe liver histopathology in mice,oil red O staining was used to observe lipid deposition,and triglyceride(TG)level in liver tissue and serum alanine aminotransferase(ALT)level were measured.In experiment 2 for exploring the effect of different extracts of Xuefu Zhuyu decoction on mice with NAFLD induced by high-fat diet,the methods of water decocting,water extraction and alcohol precipitation,and petroleum ether extraction were used to obtain the extracts 1,2,and 3 of Xuefu Zhuyu decoction,and 54 healthy male C57 BL/6 J mice were randomly divided into normal group,model group,Xuefu Zhuyu decoction extract 1,2,and 3 groups,and Xuefu Zhuyu decoction control group,with 9 mice in each group.The mice in the normal group were given control diet,and those in the other groups were given high-fat diet.Gastric administration was started at week 13,and related samples were collected at the end of week 16.Food intake and body weight were recorded,and enzyme-linked immunosorbent assay was used to measure the serum level of fasting insulin,fasting blood glucose was measured,and insulin resistance index was calculated.HE and NAS
作者
唐浩
尹艺晓
刘伟
方怡
王君
胡义扬
彭景华
TANG Hao;YIN Yixiao;LIU Wei;FANG Yi;WANG Jun;HU Yiyang;PENG Jinghua(Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Institute of Liver Diseases,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Key Laboratory of Liver and Kidney Diseases of the Ministry of Education,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Key Laboratory of Traditional Chinese Clinical Medicine,Shanghai 201203,China)
出处
《临床肝胆病杂志》
CAS
北大核心
2022年第12期2728-2737,共10页
Journal of Clinical Hepatology
基金
国家自然科学基金面上项目(81673750)
上海中医药大学附属曙光医院四明学者基金(SGXZ-201911)。
