摘要
目的分析云南省2021年手足口病(Hand,foot,and mouth disease,HFMD)病例埃可病毒18型(Echovirus 18,E18)分离株基因特征。方法从2021年云南省2个市州HFMD病例粪便标本中分离E18;对VP1区基因进行扩增、测序和拼接,获得全序列;构建E18分离株与GenBank中参考株的系统进化树,分析基因特征。结果从HFMD病例中获得8株E18分离株,其完整VP1区序列的核苷酸、氨基酸同源性分别为96.75%-99.77%、97.21%-100%;与原型株Metcalf相比的核苷酸、氨基酸同源性分别为77.24%-78.63%、91.99%-93.73%。基于VP1区分型,8株分离株均属于基因型5分支2,且在VP1区存在特有的氨基酸位点变异(T80A、I240V、T274S和H281R)。结论本研究E18分离株属于基因型5,可能在环境中循环了一段时间。需进一步加强HFMD病例E18监测和分子生物学特征研究。
Objective To analyze genetic characteristics of echovirus 18(E18)isolated from hand,foot and mouth disease(HFMD)cases in Yunnan province in 2021.Methods We isolated E18 from stool specimens of HFMD cases in two prefectures of Yunnan in 2021.We amplified,sequenced,and spliced E18 VP1 region genes to obtain complete sequences.We constructed a phylogenetic tree of E18 isolates and GenBank reference strains to determine genetic characteristics.Results Eight E18 strains were obtained from HFMD cases;nucleotide and amino acid homologies in the complete VP1 regions were 96.75%-99.77% and 97.21%-100%,respectively.Compared with the prototype strain,Metcalf,nucleotide and amino acid homologies were 77.24%-78.63% and 91.99%-93.73%.Based on VP1 phylogenetic analysis,the eight isolates belonged to branch 2 of genotype 5,with specific amino acid mutations of T80 A,I240 V,T274 S,and H281 R in VP1 regions.Conclusions The E18 strains in the study belonged to genotype 5,which may have circulated in the environment for some time.We need to further monitor E18 and its molecular characteristics in HFMD cases.
作者
刘艳艳
杨善蕊
段忠文
姜黎黎
伏晓庆
张勇
Liu Yanyan;Yang Shanrui;Duan Zhongwen;Jiang Lili;Fu Xiaoqing;Zhang Yong(Kunming Municipal Center for Disease Control and Prevention,Kunming 650228,Yunnan,China;Dehong Prefectural Center for Disease Control and Prevention,Mangshi 678400,Yunnan,China;Yunnan Provincial Center for Disease Control and Prevention,Kunming 650022,Yunnan,China)
出处
《中国疫苗和免疫》
CSCD
北大核心
2022年第5期503-508,共6页
Chinese Journal of Vaccines and Immunization
基金
昆明市卫生科技人才培养项目暨“十百千”工程(No.2019-sw(后备)-51)。
关键词
手足口病
埃可病毒18型
基因特征
Hand
foot and mouth disease
Echovirus 18
Genetic characteristics
