摘要
目的探讨睾丸生精障碍患者的染色体异常及多态性特点。方法选取2020年4月~2021年5月经本院生殖医学科室诊断为睾丸生精障碍的295例患者作为研究对象,其中少精子症178例,无精子症117例,进行染色体G显带核型分析,化学发光法试剂盒检测血清性激素。结果本研究纳入的睾丸生精障碍患者295例,染色体异常为75例(21.13%),少精子症178例中染色体异常为35例(19.66%),无精子症为117例,其中染色体异常为40例(34.19%),比较有差异(X~2=7.856,P=0.005)。295例患者中染色体多态性71例(24.07%),少精子症178例中染色体多态性为34例(19.10%),无精子症117例中染色体多态性37例(31.62%),比较有差异(X~2=6.597,P=0.010);少精子症患者的染色体核型异常主要表现为平衡易位为10例(28.57%),次要表现为臂间倒位6例(17.14%),无精子症患者的染色体核型异常主要表现为克氏征共28例(70.00%);无精子症中Y染色体多态性25例(67.57%),小Y长臂次缢痕增加4例(10.81%),随体多态性为4例(10.81%),9号染色体倒位为4例(10.81%),少精子症患者分别25例(73.53%)、2例(5.88%)、2例(5.88%)及5例(14.71%)。与少精子症相比,无精子症患者染色体核型异常及染色体多态性患者血清中FSH、LH升高,T水平降低(P<0.05)。结论男性睾丸生精障碍患者存在染色体数目异常及Y染色体多态性改变,其中以克氏征(47,XXY)较常见,与无精子症患者相比,少精子症患者血清中FSH、LH降低,T升高,可作为检测男性患者生精障碍指标并与染色体异常相关,具体机制值得深入研究。
Objective To investigate the characteristics of chromosome abnormality and polymorphism in testicular spermatogenesis disorder.Methods Total of 295 patients diagnosed as testicular sperm production obstacle in department of reproductive medicine in our hospital from April,2020 to May,2021 were taken as the research object.Of the patients,178cases were oligozoospermia and 117 cases azoospermia.Chromosome G banding karyotype and the levels serum reproductive hormones of the patients were detected and analyzed.Results Among 295 cases of testicular spermatogenic dysfunction,75cases(21.13%)had chromosomal abnormalities.Of 178 cases of oligozoospermia,35 cases(19.66%)had chromosomal abnormalities,and of 117 cases of azoospermia,40 cases(34.19%)had chromosomal abnormalities,showing a significant difference in row comparison(X2=7.856,P=0.005).Among 295 patients,71(24.07%)had chromosome polymorphism.Of178 cases of oligozoospermia,34(19.10%)had chromosome polymorphism,and of 117 cases of azoospermia,37(31.62%)had chromosome polymorphism(X2=6.597,P=0.010).In patients with oligozoospermia,10 cases(28.57%)of chromosomal karyotype abnormalities mainly exhibited balanced translocation,6 cases(17.14%)interbrachial inversion,other 28 cases(70.00%)of azoospermia mainly exhibited Klinson’s sign.In azoospermia,there were 25 cases(67.57%)of Y chromosome polymorphism,4 cases(10.81%)of increase in small Y long arm secondary hanged mark,4 cases(10.81%)of satellite polymorphism,4 cases(10.81%)of chromosome 9 inversion.There were 25(73.53%),2(5.88%),2(5.88%)and 5(14.71%)oligozoospermia patients,respectively.Compared with oligozoospermia,serum FSH and LH levels were increased and T levels were decreased in azoospermia patients with chromosomal karyotype abnormality and chromosomal polymorphism(P<0.05).Compared with the control group,the serum FSH and LH levels in azoospermia patients with chromosome karyotype abnormality and chromosome polymorphism were higher than those in the control group,while the serum T level was lower than that in
作者
李秀兰
莫小东
唐莉
魏海云
Li Xiulan;Mo Xiaodong;Tang Li;Wei Haiyun(Prenatal Diagnosis Center of Dongguan People's Hospital,Dongguan,Guangdong,523000;Department of Reproductive Medicine,Dongguan People's Hospital,Dongguan,Guangdong,523000)
出处
《中国男科学杂志》
CAS
CSCD
2022年第4期83-87,91,共6页
Chinese Journal of Andrology
关键词
精子发生
性腺甾类激素
核型分析
染色体多态性
spermatogenesis
gonadal steroid hormones
karyotyping
chromosomal polymorphism
