摘要
目的:建立蔓性千斤拔饮片的质量标准。方法:考察饮片的性状特征,采用薄层色谱法(TLC)进行定性鉴别,对水分、总灰分检查项及水溶性浸出物进行测定,采用高效液相色谱法(HPLC)测定饮片中染料木苷和染料木素的含量,色谱柱:安捷伦SB-C18(250 mm×4.6 mm,5μm),流动相:乙腈(A)-0.5%冰醋酸溶液(B),梯度洗脱,流速:1.0 mL/min,检测波长:260 nm,柱温:30℃,进样量:10μL。结果:蔓性千斤拔的性状特征明显;TLC鉴别斑点清晰,分离良好;15批蔓性千斤拔水分为6.82%~12.88%、总灰分为1.73%~3.84%、浸出物为15.09%~31.58%;染料木苷和染料木素总量为0.0439%~0.0808%。结论:该标准提升了蔓性千斤拔饮片质量的可控制性。
Objective:To establish the quality standard of Flemingia philippinensis.Methods:To investigate the characteristics of the decoction pieces,thin layer chromatography(TLC)was used for qualitative identification,and the moisture,total ash examination items and water-soluble extracts were determined.The contents of genistin and genistein in the decoction pieces were determined by high performance liquid chromatography(HPLC).The chromatographic column:Agilent SB-C18(250 mm×4.6 mm,5μm),mobile phase:acetonitrile(A)-0.5%glacial acetic acid solution(B),gradient elution,flow rate:1.0 mL/min,detection wavelength:260 nm,column temperature:30℃,injection volume:10μL.Results:The characters of Flemingia philippinensis were obvious.TLC identification spots are clear and well separated.The moisture content of 15 batches of Flemingia philippinensis is between 6.82%-12.88%,the total ash content is between 1.73%-3.84%,the extract is between 15.09%-31.58%,and the total amount of genistin and genistein is between 0.0439%-0.0808%.Conclusion:This standard improves the controllability of the quality of Flemingia philippinensis decoction pieces.
作者
佘永红
杨德泉
丁野
SHE Yonghong;YANG Dequan;DING Ye(Food and Drug Inspection Institute of Xiangxi Tujia and Miao Autonomous Prefecture,Jishou Hunan,416000,China;Hunan Institute of drug inspection and testing,Changsha Hunan 410001,China)
出处
《药品评价》
CAS
2022年第18期1116-1120,共5页
Drug Evaluation
基金
湖南省自然科学基金项目(2021JJ80030)。
关键词
医学
传统
少数民族
豆科
蔓性千斤拔
饮片
质量标准
鉴别方法
检查项目
水溶性浸出物
含量测定
Medicine,traditional
Nationalminority
Fabaceae
Flemingia philippinensis
Decoction pieces
Quality standards
Identification method
Inspection items
Water-soluble extract
Content determination
