摘要
目的分析T淋巴细胞亚群(CD3^(+)、CD4^(+)、CD8^(+))、自然杀伤(NK)细胞及血小板活化指标[P-选择素(CD62p)、颗粒膜蛋白140(GMP-140)]检测在弥漫性大B细胞淋巴瘤(DLBCL)患者诊疗中的效能。方法选取诊治的59例DLBCL患者为观察组,于同期选取54例健康体检者为对照组。采集2组受试者5 mL静脉血,对T淋巴细胞亚群、NK细胞及血小板活化指标进行检测比较;同时绘制受试者操作特征(ROC)曲线,探究各项指标单独与联合检测诊断DLBCL的效能。结果观察组CD3^(+)(52.64±5.21)%、CD4^(+)(35.42±3.67)%、NK细胞水平(9.45±1.22)个·L^(-1),低于对照组的(67.75±6.48)%、(49.46±4.35)%、(18.67±1.64)个·L^(-1),CD8^(+)、CD62p、GMP-140(28.67±3.44)%、(2.58±0.67)%、(14.59±2.31)%,高于对照组的(20.59±2.26)%、(1.59±0.45)%、(8.02±1.54)%,差异有统计学意义(P<0.05)。ROC曲线结果显示,T淋巴细胞亚群、NK细胞及血小板活化指标联合检测在DLBCL诊断中的曲线下面积(AUC)为0.984(95%CI:0.967~1.000),高于各项指标单独检测[0.730(95%CI:0.637~0.823)、0.648(95%CI:0.525~0.770)、0.856(95%CI:0.782~0.929)、0.821(95%CI:0.734~0.908)、0.875(95%CI:0.808~0.942)、0.834(95%CI:0.762~0.906)]。结论T淋巴细胞亚群、NK细胞及血小板活化指标在DLBCL患者血清内呈异常表达,各项指标联合检测效能较高,可为DLBCL早期诊治提供可靠的参考依据,指导临床制定科学规范的治疗手段,提高患者生存率。
Objective To analyze the efficacy of T lymphocyte subsets(CD3^(+),CD4^(+),CD8^(+)),natural killer(NK)cells and platelet activation indexes[P-selectin(CD62p),granular membrane protein 140(GMP-140)]in the diagnosis and treatment of patients with diffuse large B-cell lymphoma(DLBCL).Methods 59 DLBCL patients diagnosed and treated were selected as the observation group,and 54 healthy subjects were selected as the control group during the same period.5 mLvenous blood was collected from the two groups,and the T lymphocyte subsets,NK cells and platelet activation indexes were detected and compared;At the same time,the receiver operating(ROC)curve was drawn to explore the efficacy of individual and combined detection and diagnosis of each index in the diagnosis of DLBCL.Results CD3^(+)(52.64±5.21)%,CD4^(+)(35.42±3.67)%,NK cell level(9.45±1.22)·L^(-1) in the observation group were lower than those in the control group(67.75±6.48)%and(49.46±4.35)%,(18.67±1.64)L^(-1),CD8^(+),CD62p,GMP-140(28.67±3.44)%,(2.58±0.67)%,(14.59±2.31)%,higher than the control group(20.59±2.26)%,(1.59±0.45)%,(8.02±1.54)%,and the above results a statistically significant(P<0.05).The ROC curve results showed that the area under the curve(AUC)of the combined detection of T lymphocyte subsets,NK cells and platelet activation indicators in the diagnosis of DLBCL was 0.984(95%CI:0.967 to 1.000),which was higher than the detection of each indicator alone[0.730(95%CI:0.637 to 0.823),0.648(95%CI:0.525 to 0.770),0.856(95%CI:0.782 to 0.929),0.821(95%CI:0.734 to 0.908),0.875(95%CI:0.808 to 0.942),0.834(95%CI:0.762 to 0.906)].Conclusion T lymphocyte subsets,NK cells and platelet activation indexes are abnormally expressed in the serum of patients with DLBCL.The combined detection of these indexes has a high detection efficiency,which can provide a reliable reference for the early diagnosis and treatment of DLBCL,and guide the clincial formulation of seientific and standardized treatment methods to improve the survival rate of patients.
作者
栗朋辉
LI Penghui(Department of Laboratory,the Second People’s Hospital of Luohe City,Henan Luohe 462000,China)
出处
《河南医学高等专科学校学报》
2022年第5期566-571,共6页
Journal of Henan Medical College
