摘要
目的 探讨IP10基因rs3921及rs4386624位点多态性与川崎病(KD)的相关性。方法 选取2017年3月至2020年12月咸宁市中心医院确诊的80例KD患儿为病例组,根据有无心血管疾病再分为冠状动脉病变(CAL)组、无冠状动脉病变(NCAL)组,分别有32、48例;同时选择67例健康儿童作为对照组。采用多重聚合酶链式反应(PCR)测定上述各组IP10 rs3921、rs4386624各基因型突变型、各等位基因突变频率。结果 (1)病例组IP10 rs3921 AA基因型频率、IP10 rs3921 A等位基因频率均高于对照组,χ^(2)值分别为34.066、20.254,P<0.05;IP10 rs3921 AA基因型、IP10 rs3921 A等位基因频率能增加KD的易感性,OR(95%CI)值分别为8.62(4.01~18.53)、2.93(2.25~3.90);(2)CAL组IP10 rs3921 AA基因型频率、IP10 rs3921 A等位基因频率均高于NCAL组,χ^(2)值分别为23.254、18.965,P<0.05;IP10 rs3921 AA基因型、IP10 rs3921 A等位基因频率能增加CAL的易感性,OR(95%CI)值分别为9.51(3.84~19.86)、2.49(1.23~4.86);(3)病例组IP10 rs4386624 AA基因型频率、IP10 rs4386624 A等位基因频率均高于对照组,χ^(2)值分别为22.015、18.547,P<0.05;IP10 rs4386624 AA基因型、IP10 rs4386624 A等位基因频率能增加KD的易感性,OR(95%CI)值分别为13.70(5.15~24.21)、5.65(3.42~9.85);(4)CAL组IP10 rs4386624 AA基因型频率、IP10 rs4386624 A等位基因频率均高于NCAL组,χ^(2)值分别为13.269、22.214,P<0.05;IP10 rs4386624 AA基因型、IP10 rs4386624 A等位基因频率能增加CAL的易感性,OR(95%CI)值分别为8.54(3.14~17.31)、3.55(2.56~6.60)。结论 IP10基因rs3921、rs4386624位点多态性与KD及其并发症CAL有关,携带AA基因型可能使KD及其并发CAL风险增加。
Objective To investigate relationship between polymorphisms of IP10 gene loci rs3921 and rs4386624 and Kawasaki disease(KD).Methods A total of 80 children who were diagnosed as KD in our hospital from March 2017 to December 2020 were selected as case group.According to presence or absence of cardiovascular disease, they were divided into coronary artery lesion group(CAL group, 32 cases) and non coronary artery lesion group(NCAL group, 48 cases),and 67 healthy children were selected as control group.Multiplex PCR was used to determine mutation frequencies of genotypes and alleles of IP10 gene loci rs3921 and rs4386624 of the children with KD in the two groups.Results(1)The frequency of IP10 rs3921 AA genotype of the children in the case group was higher than that in the control group(67.50% vs 19.40%)(χ^(2)=34.066,P<0.05),and the IP10 rs3921 AA genotype could increase the susceptibility to KD(OR=8.62,95%CI:4.01-18.53).The frequency of IP10 rs3921 A allele of the children in the case group was higher than that in the control group(74.37% vs 23.15%),and the IP10 rs3921 A allele frequency could increase the susceptibility to KD(OR=2.93,95%CI:2.25-3.90)(χ^(2)=20.254,P<0.05).(2)The frequency of IP10 rs3921 AA genotype of the children in CAL group was higher than that in NCAL group(87.50% vs 54.17%)(χ^(2)=23.254,P<0.05),and the IP10 rs3921 AA genotype could increase the susceptibility to CALs(OR=9.51,95%CI:3.84-19.86).The frequency of IP10 rs3921 A allele of the children in CAL group was higher than that in NCAL group(93.75% vs 61.46%)(χ^(2)=18.965,P<0.05),and the IP10 rs3921 A allele frequency could increase the susceptibility to CALs(OR=2.49,95%CI:1.23-4.86).(3)The frequency of IP10 rs4386624 AA genotype of the children in the case group was higher than that in the control group(77.50% vs 46.30%)(χ^(2)=22.015,P<0.05),and the IP10 rs4386624 AA genotype could increase the susceptibility to KD(OR=13.70,95%CI:5.15-24.21).The frequency of IP10 rs4386624 A allele of the children in the case group was higher than that i
作者
张芳霞
龚育红
王立琼
ZHANG Fangxia;GONG Yuhong;WANG Liqiong(Department of Internal Pediatrics,Xianning Municipal Central Hospital/The First Affiliated Hospital of Hubei College of Science and Technology,Hubei Xianning 437100,China)
出处
《中国妇幼健康研究》
2022年第11期18-23,共6页
Chinese Journal of Woman and Child Health Research
