摘要
目的 观察miR-1826在前列腺癌(PC)及其细胞株中的表达,并探讨其对PC-3、DU145细胞侵袭和迁移能力的影响。方法 实时定量PCR(qRT-PCR)研究miR-1826在前列腺癌组织、癌旁正常组织及PC-3、DU145细胞的表达。采用Kaplan-Meier曲线分析miR-1826的表达与PC患者生存率的关系。采用Cox回归分析研究影响PC患者预后的危险因素。用miR-1826模拟物、模拟物阴性对照(NC)、miR-1826抑制剂或抑制剂NC转染PC细胞。通过CCK-8法和集落形成试验研究miR-1826对PC细胞增殖的影响,通过Transwell实验检测miR-1826对PC细胞迁移和侵袭能力的影响。结果 miR-1826在PC组织中的表达低于邻近正常组织,两种PC细胞系中miR-1826的表达水平均低于正常人前列腺上皮细胞系。与高表达患者相比,miR-1826低表达患者的总生存期较短。miR-1826的下调可以促进PC细胞的迁移和侵袭。结论 miR-1826的低表达可能促进了PC的进展,上调miR-1826能抑制PC细胞的侵袭、迁移能力。
Objective To observe the expression of miR-1826 in prostate cancer(PC) tissues and cells, and to explore the effects of miR-1826 on invasion, migration of PC-3,DU145 cells.Methods The expression of miR-1826 in PC tissues and adjacent tissues as well as in cell lines PC-3,DU145,was detected by qRT-PCR.Kaplan-Meier curves were used to analyze the relationship between the expression of miR-1826 and the survival rate of PC patients.Cox regression analysis was used to study the risk factors affecting the prognosis of PC patients.PC cells were transfected with miR-1826 mimic, mimic negative control(mimic NC),miR-1826 inhibitor, or inhibitor NC.The effect of miR-1826 on the proliferation of PC cells was studied by the CCK-8 method and colony formation assay.Transwell assays were used to detect the effect of miR-1826 on the migratory and invasive abilities of tumor cells.Results The expression of miR-1826 in PC tissues was lower than that in adjacent normal tissues, and that the expression levels of miR-1826 in two PC cell lines were all lower than normal human prostate epithelial cells line.Patients with low expression of miR-1826 had shorter overall survival compared with those with high expression.The downregulation of miR-1826 promoted PC cell proliferation, migration, and invasion.Conclusion The expression of miR-1826 decreases in PC tissues, and up-regulation of miR-1826 can inhibit the invasion and migration of PC cells.
作者
韩献成
刘静
刘永国
HAN Xiancheng;LIU Jing;LIU Yongguo(Department of Urology,the Affiliated Hospital of Weifang Medical University,Weifang 261031,China;Department of Pathology,Weifang Traditional Chinese Hospital)
出处
《潍坊医学院学报》
2022年第4期272-275,共4页
Acta Academiae Medicinae Weifang
