摘要
目的探讨胃癌患者幽门螺旋杆菌(HP)感染程度与程序性细胞死亡蛋白1(PD-1)及其配体PD-L1的表达水平的相关性,为临床治疗提供参考。方法前瞻性选取2019年11月至2021年11月长沙市第一医院收治的胃癌患者118例,根据HP感染程度分为轻度组42例,中度组40例和重度组36例。采用实时荧光定量检测PD-1和PD-L1表达水平,采用Spearman相关分析胃癌患者HP感染程度与PD-1和PD-L1表达水平间的相关性,采用有序logistic回归筛选影响HP感染程度的因素。结果三组间PD-L1、PD-1、胃癌病程、吸烟史比较差异有统计学意义(P<0.05)。相关分析显示,HP感染程度与PD-1、PD-L1表达水平呈正相关(r=0.546,0.582,P<0.05)。有序logistic回归显示,高表达的PD-L1(OR=2.004,95%CI:1.726~2.326)、PD-1(OR=3.347,95%CI:2.694~4.158),胃癌病程长(OR=3.007,95%CI:2.547~3.550)和有吸烟史(OR=1.634,95%CI:1.547~2.431)是胃癌患者HP感染程度的危险因素。结论胃癌患者HP感染程度与PD-1、PD-L1表达呈正相关,且高表达PD-1和PD-L1是HP感染严重程度的危险因素。
Objective To explore the correlation between the degree of Helicobacter pylori(HP)infection and the expression levels of programmed death-1(PD-1)and its ligand PD-L1 in gastric cancer patients.Methods A total of 118 patients with gastric cancer who were treated in Changsha First Hospital from November 2019 to November 2021 was selected prospectively and divided into mild group(n=42),moderate group(n=40)and Severe group(n=36)according to the degree of HP infection.Real-time fluorescence quantitative detection was used to detect the expression levels of PD-1,PD-L1,and Spearman correlation was used to analyze the correlation between the degree of HP infection and the expression levels of PD-1 and PD-L1.The factors affecting the degree of HP infection were screened by ordinal logistic regression.Results There were significant differences in the expression levels of PD-L1 and PD-1,the course of gastric cancer,and smoking history among the three groups(P<0.05).Spearman correlation analysis showed that the degree of HP infection was positively correlated with the expression levels of PD-1 and PD-L1(r=0.546,r=0.582,P<0.05).The ordinal logistic showed PD-L1(OR=2.004,95%CI:1.726-2.326),PD-1(OR=3.347,95%CI:2.694-4.158),gastric cancer course(OR=3.007,95%CI:2.547-3.550),with smoking history(OR=1.634,95%CI:1.547-2.431)were risk factors for the degree of HP infection in patients with gastric cancer(P<0.05).Conclusion The degree of HP infection in gastric cancer patients was positively correlated with the expression of PD-1 and PD-L1,and PD-1 and PD-L1 were risk factors for the degree of HP infection.
作者
彭进
王伟宁
罗文
谭智
叶冠男
周震
PENG Jin;WANG Wei-ning;LUO Wen;TAN Zhi;YE Guan-nan;ZHOU Zhen(Department of Gastroenterology,Changsha First Hospital,Changsha,Hunan 410000,China)
出处
《中国临床研究》
CAS
2022年第11期1565-1569,共5页
Chinese Journal of Clinical Research
基金
湖南省卫生健康委科研计划课题(B2019134)。
