摘要
浮游植物是湖库生态系统物质循环和能量流动的主要参与者,了解其群落组成对保护水生生态系统平衡具有重要意义.2021年6月在南水北调中线工程水源地丹江口水库淅川库区设置7个采样点,分4层分别在0.5、5、10和20 m处共采取28个样品.采用高通量测序测定真核浮游植物群落结构,分析真核浮游植物群落在水平和垂向上的分布特征.采用冗余分析探讨了浮游植物主要类群与环境因子的关系.结果表明:(1)库区真核浮游植物共12门68属,物种为甲藻-硅藻-绿藻型结构.水平方向上浮游植物群落差异较大,各采样点垂直方向上浮游植物丰度和多样性存在较大差异.宋岗、土门和党子口浮游植物群落多样性和丰度随水深增加呈下降趋势,其他采样点的浮游植物群落与水深关系趋势不明显.(2)硝氮(NO_(3)^(-)-N)、水深、溶解氧(DO)、pH和水温(WT)是影响库区浮游植物垂直分布的重要环境因子.环境因子对浮游植物门类影响各异,甲藻门与NO_(3)^(-)-N和水深呈负相关,与其他环境因子呈正相关;硅藻门与NO~--N和水深呈正相关,与其他环境因子呈负相关;绿藻门与WT、pH和DO呈负相关,与水深无明显相关性.
Eukaryotic phytoplankton play crucial roles in ecosystem processes,and understanding their community composition and abundance is of great importance to protect the balance of aquatic ecosystems.Danjiangkou Reservoir is a canyon lake in central China that acts as the water source of the Mid-route of South-to-North Water Diversion Project.In this study,the composition of planktonic microeukaryotes from water samples with a depth ranging from 0.5 to 20 m in Danjiangkou Reservoir were investigated using high-throughput 18 S rDNA gene sequencing.The environmental factors including pH,water temperature(WT),nitrate(NO-N),ammonia(NH-N),total nitrogen(TN),conductivity(Cond.),water depth,and dissolved oxygen(DO)in the reservoir areas were measured,and their correlations with the abundance and diversity of eukaryotic phytoplankton were analyzed.The results of high-throughput sequencing showed that a total of 68 genera of phytoplankton belonging to 12 phyla were detected,and the phytoplankton communities were mainly composed of Dinophyceae,Bacillariophyta,and Chlorophyta.In particular,significant differences in the diversity of phytoplankton communities on a vertical distribution were found.The diversity and abundance of the phytoplankton communities in Songgang(SG),Tumen(TM),and Dangzikou(DZK)decreased with the increase in water depth,and the relationship between phytoplankton and water depth in other sampling sites was not obvious.The results of RDA showed that NO_(3)^(-)-N,water depth,DO,pH,and WT could influence the vertical distribution of the phytoplankton community in the Danjiangkou Reservoir.Among these phytoplankton types,Dinophyceae was negatively correlated with NO_(3)^(-)-N and water depth and positively correlated with other environmental factors.Meanwhile,Bacillariophyta was positively correlated with NO-N and water depth and negatively correlated with other environmental factors.In addition,Chlorophyta was negatively correlated with WT,pH,and DO and not significantly correlated with water depth.
作者
贺玉晓
买思婕
任玉芬
李卫国
赵同谦
马寅男
HE Yu-xiao;MAI Si-jie;REN Yu-fen;LI Wei-guo;ZHAO Tong-qian;MA Yin-nan(Institute of Resources and Environment,Henan Polytechnic University,Jiaozuo 45400,China;State Key Laboratory of Urban and Regional Ecology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Daning Management Office of Beijing South to North Water Diversion,Beijing 100442,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2022年第11期5096-5105,共10页
Environmental Science
基金
国家自然科学基金项目(U1704241)
中原科技创新领军人才项目(194200510010)。
关键词
丹江口水库
浮游植物
群落结构
环境因子
高通量测序
Danjiangkou Reservoir
phytoplankton
community structure
environmental factor
high-throughput sequencing
