摘要
目的对2011年和2017年分别在我国甘肃省和海南省分离的两株盖塔病毒(GS11-155和HNDZ1712-1)进行全基因组核苷酸序列测定,分析其与我国1964年首次分离的盖塔病毒(M1)的分子差异及分子遗传进化特征。方法使用病毒基因扩增技术测定新分离的两株盖塔病毒全基因组核苷酸序列,建立盖塔病毒基因组数据集并使用生物信息学软件进行病毒分子特征及分子遗传进化分析等。结果两株新分离盖塔病毒(GS11-155和HN⁃DZ1712-1)基因组全长分别为11690 nt和11621 nt。两株病毒均具有甲病毒基因组结构特征。虽然两株病毒的结构基因、非结构基因以及非编码的连接区核苷酸序列长度均完全相同,但是病毒基因组5'和3'非编码区的核苷酸序列长度存在差异。两病毒株基因组3'UTR重复序列单元的结构未发生变化。病毒基因组同源性分析结果显示,海南省2017年分离的盖塔病毒(HNDZ1712-1)与我国于1964年首次在海南省蚊虫分离的盖塔病毒(M1)之间的核苷酸与氨基酸同源性分别为97.7%和98.1%。盖塔病毒全基因组核苷酸序列的分子遗传进化分析结果显示,本研究的两株病毒及其所属病毒株分别形成两个单独的进化簇,甘肃2011年进化簇和海南2017年进化簇,并与1964年海南首次分离的病毒形成完全独立的进化种群。结论虽然HNDZ1712-1病毒同样分离自海南岛蚊虫标本,但是与1964年海南岛分离的病毒(M1)处在完全不同的进化分支,而与数千公里之外的甘肃省分离株(GS11-155)具有较近的进化关系,提示新分离的两株盖塔病毒与1964年分离的盖塔病毒存在较大分子遗传差异。
Objective The main aim of the study is to sequence the complete genome of two Getah virus strains(GS11-155 and HNDZ1712-1)isolated in Gansu Province and Hainan Province in 2011 and 2017 respectively and analyze the molecular and genetic evolution of the two strains compared with M1,which was first isolated in 1964 in Hainan Province,China.Methods Genome of two newly isolated Getah viruses were sequenced by virus gene amplification technique,and the genomic database of Getah viruses was established.The molecular characteristics and genetic evolution of the viruses were analyzed by bioinformatics software.Results The genome length of two new isolated Getah virus strains(GS11-155 and HNDZ1712-1)was 11690 nt and 11621 nt,respectively.Both strains had the structural characteristics of Alphavirus genome.Although the nucleotide sequence lengths of structural genes,non-structural genes and non-coding junction regions of the two strains were identical,the nucleotide sequence lengths of the 5'and 3'non-coding regions of the viral genomes were a few different.The 3'UTR repeats elements in the genomes of the two virus strains did not change.It was 97.7%and 98.1%different of nucleotide and amino acid homology between both strains of Getah virus,HNDZ1712-1 isolated in 2017 and M1 isolated in 1964 in Hainan Province.Interesting,Gansu 2011 cluster and Hainan 2017 cluster were emerged leading by both strains GS11-155 and HNDZ1712-1 respectively,those two clusters totally independent with M1 virus isolated from Hainan in 1964 in whole genome phylogenetic analysis first.Conclusions Although the HNDZ1712-1 was also isolated from mosquito samples in Hainan Province,it was in a completely different evolutionary branch from the M1 isolated from Hainan Island in 1964,and was closely related to the strain isolated from Gansu Province(GS11-155)thousands of kilometers away.It is suggested that the two new strains of Getah virus are different from the Getah virus isolated in 1964.
作者
李彬
付士红
查冰
范娜
李元元
徐学平
应思超
聂凯
许松涛
李兴洲
王环宇
梁国栋
LI Bin;FU Shi-hong;ZHA Bing;FAN Na;LI Yuan-yuan;XU Xue-ping;YING Si-chao;NIE Kai;XU Song-tao;LI Xing-zhou;WANG Huan-yu;LIANG Guo-dong(School of Public Health,Jiamusi University,Jiamusi,Heilongjiang 154007,China;National Institute for Viral Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 100052,China;National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025,China)
出处
《中国热带医学》
CAS
2022年第10期916-922,共7页
China Tropical Medicine
基金
传染病预防控制国家重点实验室重点项目(No.2015SKLID505)。
关键词
盖塔病毒
分子遗传进化
人畜共患病
Getah virus
molecular genetic evolution
zoonotic virus
