摘要
目的:基于网络药理学方法探讨益气复元汤治疗肺癌的作用机制。方法:通过TCMSP平台筛选出益气复元汤的主要药物成分,通过GeneCard、OMIM、TTD数据库检索肺癌疾病靶点,使用软件Cytoscape 3.8.2建立蛋白质-蛋白质相互作用(PPI)网络,基于DAVID数据库对核心靶点进行基因本体(GO)功能与京都基因与基因组百科全书(KEGG)通路富集分析。结果:共获得益气复元汤主要活性成分131种,潜在靶点273个,肺癌相关疾病靶点13215个,药物-疾病靶点256个。PPI网络显示主要靶点有雌激素受体1、丝氨酸/苏氨酸蛋白激酶1、肿瘤坏死因子、白细胞介素6、丝裂原活化蛋白激酶1、肿瘤相关蛋白53、丝裂原活化蛋白激酶3等。GO功能主要包括药物反应、细胞缺氧反应、细胞外空隙、细胞溶质、酶结合、药物结合、蛋白结合等。KEGG通路主要有信号转导、肿瘤及癌症的特定类型、病毒相关通路等。预测丝裂原活化蛋白激酶3为益气复元汤治疗肺癌的主要靶点。结论:益气复元汤治疗肺癌具有多成分、多靶点、多通路的作用机制,其主要作用成分可能为槲皮素,作用机制可能与信号转导、肿瘤及癌症的特定类型、缺氧诱导因子-1信号通路、肿瘤坏死因子信号通路密切相关。
Objective:To investigate the mechanism of action of Yiqi Fuyuan decoction in the treatment of lung cancer based on network pharmacology.Methods:TCMSP platform was used to screen out the main components of Yiqi Fuyuan decoction,and GeneCard,OMIM,and TTD databases were used to obtain the disease targets of lung cancer.Cytoscape 3.8.2 software was used to establish a protein-protein interaction(PPI)network,and DAVID database was used to perform gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Results:A total of 131 active components were obtained for Yiqi Fuyuan decoction,as well as 273 potential targets,13215 targets associated with lung cancer,and 256 drug-disease targets.The PPI network showed that the main targets included estrogen receptor 1,serine/threonine protein kinase 1,tumor necrosis factor,interleukin-6,mitogen-activated protein kinase 1,tumor-related protein 53,and mitogen-activated protein kinase 3.GO function mainly included drug response,cell hypoxia response,extracellular space,cytosol,enzyme binding,drug binding,and protein binding,and KEGG pathways mainly included signal transduction,special types of tumor and cancer,and virus-related pathways.It was predicted that mitogen-activated protein kinase 3 was the main target for Yiqi Fuyuan decoction in the treatment of lung cancer.Conclusion:Yiqi Fuyuan decoction has the mechanism of action of multiple components,targets,and pathways in the treatment of lung cancer,and quercetin might be the main active component.Its mechanism of action may be closely associated with signal transduction,special types of tumor and cancer,hypoxia-inducible factor-1 signaling pathway,and tumor necrosis factor signaling pathway.
作者
周雅倩
卓德斌
ZHOU Yaqian;ZHUO Debin(Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;The First Affiliated Hospital of Hunan Traditional Chinese Medical College,Zhuzhou 412000,Hunan,China)
出处
《湖南中医杂志》
2022年第10期143-149,共7页
Hunan Journal of Traditional Chinese Medicine
关键词
肺癌
益气复元汤
作用机制
网络药理学
lung cancer
Yiqi Fuyuan decoction
mechanism of action
network pharmacology
