摘要
目的探究垂体同源框配对同源域转录因子1(pituitary homeobox paired homeodomain transcription 1,PITX1)、miR-556-3p在乳腺癌肝转移中的表达。方法以2019年6月~2021年6月在笔者医院诊治的142例乳腺癌患者为研究对象,其中64例乳腺癌肝转移患者为转移组,78例未发生肝转移患者为未转移组。采用免疫组化法检测PITX1表达。采用实时荧光定量PCR(qRT-PCR)法检测组织中PITX1mRNA、miR-556-3p的表达量。Pearson相关性分析PITX1与miR-556-3p的关系。多因素Logistic回归分析影响乳腺癌患者发生肝转移的危险因素。ROC曲线分析PITX1、miR-556-3p对乳腺癌患者发生肝转移的预测价值。结果乳腺癌肝转移组和未转移组患者TNM分期、淋巴结转移、组织学分级、病理类型比较,差异有统计学意义(P<0.05)。转移组患者PITX1阳性表达率32.81%显著低于未转移组65.38%(P<0.05)。与未转移组比较,转移组患者组织PITX1mRNA水平显著降低(P<0.05),miR-556-3p水平显著升高(P<0.05)。Pearson相关性分析结果显示,PITX1与miR-556-3p呈显著负相关(r=-0.436,P<0.001)。多因素Logistic回归分析显示,组织学分级3~4级、PITX1阴性是乳腺癌患者发生肝转移的危险因素(P<0.05)。受试者工作特征(receiver operator characteristic,ROC)曲线结果显示,PITX1、miR-556-3p联合预测乳腺癌患者发生肝转移的曲线下面积(area under the curve,AUC)为0.926(敏感度为90.6%,特异性为82.1%)。结论PITX1、miR-556-3p在乳腺癌肝转移患者中表达异常,二者与肝转移的发生存在密切关系,且对乳腺癌患者发生肝转移具有一定的预测价值。
Objective To investigate the expression of pituitary homeobox paired homeodomain transcription 1(PITX1)and miR-556-3p in breast cancer liver metastasis.Methods A total of 142 breast cancer patients who were diagnosed and treated in our hospital from June 2019 to June 2021 were collected as the research objects,of which 64 patients with liver metastasis from breast cancer were in the metastasis group,and 78 patients without liver metastasis were in the non-metastasis group.Immunohistochemistry was performed to measure the expression of PITX1.Quantitative real-time PCR(qRT-PCR)was performed to measure the expression levels of PITX1mRNA and miR-556-3p in tissues.Pearson correlation was used to analyze the relationship between PITX1 and miR-556-3p.Multivariate Logistic regression was used to analyze risk factors for liver metastasis in breast cancer patients.ROC curve was used to analyze the predictive value of PITX1 and miR-556-3p for liver metastasis in breast cancer patients.Results There were significant differences in TNM stage,lymph node metastasis,histological grade and pathological type between the breast cancer liver metastasis group and the non-metastasis group(P<0.05).The positive expression rate of PITX1 in the metastatic group was 32.81%,which was obviously lower than that in the non-metastatic group(65.38%)(P<0.05).Compared with the non-metastatic group,the PITX1 mRNA level in the metastatic group was obviously decreased(P<0.05),and the level of miR-556-3p was obviously increased(P<0.05).The results of Pearson correlation analysis showed that PITX1 was obviously negatively correlated with miR-556-3p(r=-0.436,P<0.001).Multivariate Logistic regression analysis showed that histological grades 3-4 and PITX1 negativity were risk factors for liver metastasis in breast cancer patients(P<0.05).The ROC curve results showed that the area under the curve(AUC)of PITX1 combined miR-556-3p to predict liver metastasis in breast cancer patients was 0.926(sensitivity:90.6%,specificity:82.1%).Conclusion PITX1 and miR-556
作者
杨硕
郭满
张浩
YANG Shuo;GUO Man;ZHANG Hao(Nanyang Central Hospital,Henan 473000,China)
出处
《医学研究杂志》
2022年第11期72-76,81,共6页
Journal of Medical Research
基金
河南省科技攻关计划项目(202102310096)。
