摘要
目的探讨外周血miR-320b表达水平对颈动脉粥样硬化斑块性质的评价价值。方法选取亳州市人民医院神经内科自2020年5月至2021年9月收治的108例颈动脉粥样硬化患者设为研究组,另选取同时期的105例健康志愿者设为对照组。测定并比较2组受试者外周血miR-320b表达水平。根据颈动脉超声检查结果将研究组患者分为无斑块组和斑块组,对比miR-320b表达水平。另根据高分辨率磁共振成像检查将斑块组患者分为不稳定斑块组与稳定斑块组,对比miR-320b表达水平,分析颈动脉粥样硬化斑块性质的影响因素及miR-320b水平对颈动脉粥样硬化不稳定斑块的评价作用。结果研究组外周血miR-320b表达水平较对照组降低,差异有统计学意义(P<0.05)。与无斑块组相比,斑块组患者外周血miR-320b表达水平显著降低,差异有统计学意义(P<0.05)。外周血miR-320b水平评估颈动脉粥样硬化斑块的受试者工作特征(ROC)曲线结果显示,曲线下面积(AUC)为0.948,敏感度、特异度分别为88.24%、97.30%。美国心脏病协会(AHA)颈动脉斑块Ⅳ~Ⅴ型、Ⅵ型患者外周血miR-320b表达水平均低于Ⅰ~Ⅱ型、Ⅲ型、Ⅶ型、Ⅷ型患者,且Ⅵ型患者miR-320b表达水平低于Ⅳ~Ⅴ型患者,差异有统计学意义(P<0.05)。和稳定组斑块相比,不稳定斑块组外周血中的miR-320b表达水平显著降低(P<0.05)。多因素Logistic回归性分析显示,总胆固醇、低密度脂蛋白胆固醇水平均是颈动脉粥样硬化不稳定斑块的危险因素(OR=1.979、2.217,P<0.05),而高密度脂蛋白胆固醇、外周血miR-320b表达水平是其保护因素(OR=0.641、0.548,P<0.05)。外周血miR-320b表达水平评估颈动脉粥样硬化不稳定斑块的ROC结果显示,AUC为0.915,敏感度76.47%,特异度92.50%。结论外周血miR-320b表达水平可能和颈动脉粥样硬化斑块性质存在一定的关系,可较好地评估斑块不稳定性。
Objective To investigate the value of the miR-320b expression level in peripheral blood in evaluating the nature of carotid atherosclerotic plaque.Methods The 108 patients with carotid atherosclerosis admitted in Neurology Department of Bozhou People’s Hospital from May 2020 to September 2021 were set as the study group,and the 105 healthy volunteers in the same period were set as the control group.The expression levels of miR-320b in peripheral blood of the 2 groups were measured and compared.The patients in the study group were divided into plaque-free group and plaque group according to the results of carotid artery ultrasound examination,and the expression level of miR-320b was compared.In addition,according to the results of high resolution magnetic resonance imaging,the patients in the plaque group were divided into unstable plaque group and stable plaque group,and the miR-320b level in the this 2 groups was compared.The influencing factors of the nature of carotid atherosclerotic plaque and the evaluation effect of miR-320b level on unstable plaque of carotid atherosclerosis were analyzed.Results The expression level of miR-320b in peripheral blood of the study group was lower than that of the control group(P<0.05).Compared with plaque-free group,the expression level of miR-320b in peripheral blood in the plaque group was significantly lower(P<0.05).The results of receiver operating characteristic(ROC)curve of miR-320b level in peripheral blood in evaluating carotid atherosclerotic plaque showed that the area under the curve(AUC)was 0.948,and the sensitivity and specificity were 88.24%and 97.30%respectively.The expression levels of miR-320b in peripheral blood of patients with carotid plaque typeⅣ-ⅤandⅥof American Heart Association(AHA)were lower than that of patients with typeⅠ-Ⅱ,typeⅢ,typeⅦand typeⅧ,and the expression level of miR-320b in typeⅥpatients was lower than that in typeⅣ-Ⅴpatients,and the difference was statistically significant(P<0.05).Compared with the stable plaque gr
作者
李强
杨淼
马龙
尤晓涵
张媛媛
明霞光
李春香
武晓芳
Li Qiang;Yang Miao;Ma Long;You Xiaohan;Zhang Yuanyuan;Ming Xiaguang;Li Chunxiang;Wu Xiaofang(Department of Neurology,Bozhou People’s Hospital,Bozhou 236800,China)
出处
《中华脑科疾病与康复杂志（电子版）》
2022年第4期234-240,共7页
Chinese Journal of Brain Diseases and Rehabilitation（Electronic Edition）
