摘要
目的探讨在晚发型子痫前期(late-onset preeclampsia,LOPE)胎盘氧化应激失衡中,糖代谢来源的抗氧化物质烟酰胺腺嘌呤二核苷酸磷酸(nicotinamide adenine dinucleotide phosphate,NADPH)的表达变化。方法选取重庆医科大学第一附属医院产科于2020年11月–2021年10月行剖宫分娩的LOPE孕妇13例,正常妊娠孕妇13例,分别收集胎盘组织。采用DCFH-DA法测定LOPE孕妇和正常妊娠孕妇胎盘组织活性氧簇(reactive oxygen species,ROS)水平。分光光度法测定LOPE组及对照组胎盘组织中NADPH、谷胱甘肽(glutathione,GSH)、葡萄糖含量,磷酸戊糖代谢途径中关键限速酶葡萄糖-6-磷酸脱氢酶(glucose-6-phosphate dehydrogenase,G6PD)、磷酸葡萄糖酸脱氢酶(phospho-gluconate dehydrogenase,PGD)的表达及活性水平。应用Western blot方法检测LOPE组及对照组胎盘组织中G6PD、PGD及糖酵解途径关键限速酶磷酸果糖激酶1(phosphofructokinase-1,PFK1)的蛋白表达变化。结果LOPE胎盘组织中ROS水平较对照组胎盘升高(P<0.05),抗氧化物质NADPH、GSH以及葡萄糖含量较对照组胎盘组织升高(P<0.05),PFK1蛋白表达在LOPE胎盘中升高(P<0.05),而G6PD、PGD活性及其蛋白表达在LOPE组与对照组中未见明显差异。结论晚发型子痫前期胎盘组织中葡萄糖发生代谢重编程,是抗氧化物质NADPH、GSH异常升高的原因之一。
Objective To study the changes in the expression of nicotinamide adenine dinucleotide phosphate(NADPH),a glucose metabolism-derived antioxidant,in late-onset preeclampsia(LOPE)placenta tissue and the correlation with oxidative stress.Methods A total of 13 normal pregnant women and 13 pregnant women with LOPE who were hospitalized in the Obstetrics Department,the First Affiliated Hospital of Chongqing Medical University and who underwent elective cesarean section between November 2020 and October 2021 were included in the study.Placenta tissues were collected from the subjects.Dichloro-dihydro-fluorescein diacetate(DCFH-DA)assay was done to determine the ROS levels in the placenta tissues of the LOPE group and the normal control group.Spectrophotometric analysis was conducted to determine the levels of NADPH,glutathione(GSH),and glucose,and the expressions and activities of glucose-6-phosphate dehydrogenase(G6PD)and phospho-gluconate dehydrogenase(PGD),key rate-limiting enzymes of the pentose phosphate pathway(PPP),in the placenta tissues of the LOPE group and the normal control group.Western blot was done to determine changes in the protein expressions of phosphofructokinase 1(PFK1),a key rate-limiting enzyme of the glycolytic pathway,G6PD,and PGD in the placenta tissues from the two groups.Results ROS levels in the placenta tissue of the LOPE group were significantly higher than those of the control group(P<0.05).The levels of NADPH and GSH,two antioxidants,and glucose in the LOPE placenta were significantly higher than those of the control group(P<0.05).The expression of PFK1 was significantly elevated in the LOPE group(P<0.05).However,there were no significant differences in the activities and protein expression of G6PD and PGD between the two groups.Conclusion Glucose metabolism reprogramming takes place in LOPE placenta tissue,which may be one of the causes of the abnormal elevation of NADPH and GSH.
作者
刘颖
彭威
漆洪波
LIU Ying;PENG Wei;QI Hong-bo(Department of Obstetrics,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;Chongqing Key Laboratory of Maternal and Fetal Medicine,Chongqing Medical University,Chongqing 400016,China;Joint International Research Laboratory of Reproduction and Development of Chinese Ministry of Education,Chongqing Medical University,Chongqing 400016,China;Department of Obstetrics and Gynecology,Women and Children's Hospital of Chongqing Medical University,Chongqing 401147,China)
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2022年第6期1028-1032,共5页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金青年科学基金项目(No.82001582)资助。
关键词
晚发型子痫前期
NADPH
GSH
氧化应激
葡萄糖代谢重编程
Late-onset preeclampsia
Nicotinamide adenine dinucleotide phosphate
Glutathione Oxidative stress
Glucose metabolism reprogramming
