摘要
目的:采用光固化技术合成掺杂氧化石墨烯(graphene oxide,GO)的甲基丙烯酸化明胶(methacrylated gelatin,GelMA)3D凝胶,评价其机械强度和生物相容性。方法:将GelMA和50、100、200μg/mL GO混合后紫外光(ultravioletray,UV)固化,使用扫描电镜(scanning electron microscope,SEM)观察水凝胶结构,万能测试仪压力实验计算弹性模量,CCK-8及死/活细胞染色检测大鼠骨髓源性间充质干细胞(rat bone marrow-derived mesenchymal stem cells,rBMSC)的增殖活性。采用SPSS 23.0软件包对数据进行统计学分析。结果:GelMA/GO复合凝胶具有高度多孔结构。加入GO可提高GelMA的机械性能,GelMA/100μg组及GelMA/200μg组弹性模量显著增强,GelMA/100μg组吸水率增加,GelMA/200μg组体积膨胀率减小。当GO浓度为50μg/mL时,rBMSCs黏附与增殖能力最强(P<0.05);当GO浓度≥100μg/mL时,r BMSCs的活性降低。结论:合适浓度的GO可提高GelMA的机械性能和生物相容性。鉴于优先考虑生物性,建议50μg/mL GO/GelMA用于骨组织工程。
PURPOSE:To construct graphene oxide/methacrylated gelatin(GO/GelMA)hydrogel and to investigate its mechanical property and biocompatibility.METHODS:50,100,200μg/mL GO was added to GelMA and mixed thoroughly.The micromorphology of hydrogels was observed under scanning electron microscope(SEM).Compression text was used to assess lastic modulus,CCK-8 and live/dead cell staining was conducted to assess the viability of rat bone marrow-derived mesenchymal stem cells(r BMSCs).The data were analyzed using SPSS 23.0 software package.RESULTS:SEM showed a highly porous structure of the hydrogel.After adding GO,the elastic modulous increased(100,200μg/mL),swelling ratio increased(100μg/mL)and expansion ratio decreased(200μg/mL).The viability increased in 50μg/mL GO and decreased in 100 and 200μg/mL GO.CONCLUSIONS:Proper concentration of GO can increase the mechanical property and biocompatibility.50μg/mL GO/GelMA was suggested in bone tissue engineering given priority to biocompatibility.
作者
鲍菁
秦尉
朱青
钱文昊
BAO Jing;QIN Wei;ZHU Qing;QIAN Wen-hao(Shanghai Xuhui District Dental Center,Shanghai 200032;Department of Prosthodontics,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,National Center for Stomatology,College of Stomatology,Shanghai Jiao Tong University,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011;Shanghai United Family Hospital,Shanghai 200336,China)
出处
《上海口腔医学》
CAS
北大核心
2022年第5期491-496,共6页
Shanghai Journal of Stomatology
基金
徐汇区医学科研项目(SHXH201739)。
关键词
光固化水凝胶
纳米材料
机械性能
生物相容性
Phote-cure hydrogel
Nanomaterial
Mechanical property
Biocompatibility
