摘要
福建省某规模化养猪场出现群体腹泻病例。为明确引发疫病的病原和感染情况,采集各阶段猪群的粪便、口腔拭子和产房环境、人员外表(衣服和鞋底)等样本,利用荧光定量PCR方法进行了病毒载量检测。结果显示,引发本起疫病的病原为猪轮状病毒,且病毒污染面广,在各阶段猪群中均有检出,人员外表检出率高达87.50%,人员机械带毒可能是主要的传播途径。采取疫苗免疫、消毒和粪便管理等措施后,病情趋于稳定,未再出现腹泻病例。本病例的确诊与防治为猪场科学防控猪轮状病毒感染提供了参考。
A case of collective diarrhea was detected in a large-scale farm in Fujian Province. In order to identify the pathogen causing the disease and the status of infection,feces and oral swabs of pigs at different growth stages,as well as samples from environment in delivery rooms and personnel appearance(clothing and shoe soles)were collected for detecting the viral load by fluorescent quantitative PCR. The results showed that the disease was caused by porcine rotavirus(PoRV)that widely contaminated and was detected in all stages of pig population,the detection rate of personnel appearance was 87.50%,so it was likely to spread via the virus carried by personnel. The disease was controlled by a series of measures including vaccination,disinfection and management of feces,and no new case had occurred. A reference was therefore provided for scientific prevention and control of porcine rotavirus infection in farms.
作者
陈长福
Chen Changfu(Longyan Center for Animal Disease Prevention and Control,Longyan,Fujian 364000,China)
出处
《中国动物检疫》
CAS
2022年第12期36-39,共4页
China Animal Health Inspection
基金
福建省龙岩市科技计划项目(2020LYF9020)。
关键词
猪轮状病毒
腹泻
荧光定量PCR
诊治
防控
porcine rotavirus
diarrhea
fluorescence quantitative PCR
diagnosis and treatment
prevention and control
