摘要
目的探讨Orai1蛋白及其mRNA在正常宫颈组织、宫颈息肉组织、宫颈鳞状细胞癌组织中的表达。方法应用免疫组织化学染色法、免疫印迹法、酶联免疫吸附实验法及实时定量反转录聚合酶链反应法检测Orai1蛋白及其mRNA在正常宫颈组织、宫颈息肉组织、不同分级的宫颈鳞状细胞癌组织中的表达,所得数据用统计软件进行分析。结果Orai1蛋白及其mRNA在宫颈息肉(蛋白表达量为0.43μg/ml;mRNA相对表达量为2.64)、正常宫颈组织(蛋白表达量为0.23μg/ml;mRNA相对表达量为1.90)和宫颈鳞状细胞癌组织中的表达依次减少,并且其表达量随着该肿瘤的分级升高而下降(高、中及低分化宫颈鳞状细胞癌组织中蛋白表达量分别为0.14μg/ml、0.08μg/ml及0.02μg/ml;高、中及低分化宫颈鳞状细胞癌组织中mRNA相对表达量分别为1.32、0.58及0.18)。结论Orai1的表达变化与宫颈鳞状细胞癌的发生、发展及侵袭性相关,该分子有望成为这种恶性肿瘤早期诊断和治疗的新靶点。
Objective To investigate the expressions of Orai1 protein and its mRNA in normal cervical tissue,cervical polypous tissue and cervical squamous cell carcinoma tissue.Methods The expressions of Orai1 protein and its mRNA in normal cervical tissue,cervical polypous tissue and cervical squamous cell carcinoma tissue were detected using immunohistochemical staining,immunoblotting,enzymelinked immunosorbent assay and real-time quantitative reverse transcription polymerase chain reaction.And the obtained data were analyzed by statistical software.Results The expressions of Orai1 protein and its mRNA were reduced in turn in cervical polypous tissue(the expres⁃sion of protein:0.43μg/ml;the relative expression of mRNA:2.64),normal cervical tissue(the expression of protein:0.23μg/ml;the relative expression of mRNA:1.90)and cervical squamous cell carcinoma tissue.And their expressions were also decreased with the increa⁃ses of tumor grades(the expressions of protein in well,moderately and poorly differentiated cervical squamous cell carcinoma tissues:0.14μg/ml,0.08μg/ml and 0.02μg/ml,respectively;the relative expressions of mRNA in well,moderately and poorly differentiated cervical squamous cell carcinoma tissues:1.32,0.58 and 0.18,respectively).Conclusion The changes of Orai1 expression are related to the occurrence,progression and invasion of cervical squamous cell carcinoma,and the molecule may become a promising new target in the early diagnosis and treatment of this malignant tumor.
作者
兰晶
任国强
戴飞
LAN Jing;REN Guo-Qiang;DAI Fei(Department of Gynecology and Obstetrics,Huashan Hospital North of Fudan University,Shanghai,201907,China)
出处
《中国妇幼保健》
CAS
2022年第20期3838-3841,共4页
Maternal and Child Health Care of China
