摘要
目的建立HPLC法测定法匹拉韦有关物质的方法。方法采用Venusil XBP C18(250 mm×4.6 mm,5μm)色谱柱;流动相A为0.15%磷酸二氢铵溶液,流动相B为甲醇,梯度洗脱;流速:1.0 ml·min^(-1);检测波长:230和290 nm;柱温:30℃;进样量:10μl。结果法匹拉韦与有关物质能很好地分离,所建立的有关物质检查方法经验证,专属性、重复性及稳定性均良好。结论本研究建立的方法快速准确、重现性好,可用于法匹拉韦有关物质的测定。
Objective To establish an HPLC method for determination of related substances in favipiravir.Methods Venusil XBP C18(250 mm×4.6 mm,5μm)was adopted.Mobile phase A consisted of 0.15%ammonium dihydrogen phosphate buffer solution,while mobile phase B was composed of methanol at a flow rate of 1.0 ml·min^(-1).Results The column temperature was 30℃,and the detection wavelength was 230 and 290 nm respectively.Conclusion This method is highly sensitive,specific,accurate and repeatable,so it is a proper method for quality control of favipiravir.
作者
张东娜
宋亚彬
王保刚
窦媛媛
徐力昆
王洪权
ZHANG Dong-na;SONG Ya-bin;WANG Bao-gang;DOU Yuan-yuan;XU Li-kun;WANG Hong-quan(Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China)
出处
《解放军药学学报》
CAS
2022年第3期189-192,208,共5页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家科技重大专项资助项目,2015ZX09102022。
