摘要
目的:探讨慢性粒细胞白血病(CML)患者不同阶段染色体核型变化对疾病诊断、治疗、进展及预后的意义。方法:回顾性选取2019年7月至2021年12月就诊于空军军医大学第一附属医院的167例初诊CML患者,抽取骨髓,采用24h短期培养法制备染色体标本,然后用R显带技术进行染色体核型分析,观察其遗传学特点及意义,分析染色体变异易位及附加染色体异常与经典的Ph+CML在疾病的诊断、治疗中的差异。结果:167例CML患者染色体分析中,无分裂相患者7例(4.2%),Ph+患者154例(92.2%),Ph-患者6例(3.6%)。154例Ph+患者中,126例(81.8%)为典型Ph阳性,28例(18.2%)为变异易位或伴有附加染色体异常,涉及+8、+Ph、-Y、der(17)、del(3p)、del(15q)、del(11p)、add(21q)、add(2q)等,以+8、+Ph、-Y、der(17)为主。结论:在CML染色体核型分析中,Ph染色体是其遗传学标志,染色体核型复杂程度与CML治疗、进展及预后之间有密切联系,染色体的复杂程度越高,患者预后可能越差。
Objective:To explore the significance of chromosomal karyotype changes at different stages in the diagnosis,treatment,progression and prognosis of chronic myeloid leukemia(CML).Methods:From July 2019 to Deceber 2021,167 newly diagnosed CML patients were treated in The First Affiliated Hosptital of Air Force Military Medical University.Chromosome preparation was made using 24 hours short-term culture of bone marrow cells,and R-banding technique was used for chromosome karyotype analysis.The genetic characteristics and significance of the disease were observed.And an analysis was conducted of the differences between chromosomal variant translocations and additional chromosomal abnormalities and classical Ph+CML in the diagnosis and treatment of the disease.Results:In the 167 cases of CML patients,104 cases was male,63 cases was female,and the age range was between 11 and 86.By analyzing the karyotype of 167 cases CML pateints,7 cases(4.2%)were non mitotic,154 cases were Ph chromosome positive(Ph+)and 6 cases were Ph chromosome negative(Ph-).In the 154 cases of Ph+pateints,126 cases were classical Ph+and 28 cases were variant rearrangements or additional chromosome abnormalities,include+8,+Ph,-Y,der(17),del(3p),del(15q),del(11p),add(21q),add(2q)and so on,the majority were+8,+Ph,-Y,der(17).Conclusion:In the chromosome karyotypic analysis of CML,Ph chromosome was the genetic marker.The complexity of chromosome karyotype is closely related to CML treatment,progress and prognosis.The more complexity of the chromosomes,the worse prognosis of the patient.
作者
辛晓丽
苏小丽
高山
唐海龙
崔文静
高广勋
XIN Xiaoli;SU Xiaoli;GAO Shan(The First Affiliated Hosptital of Air Force Military Medical University,Shaanxi Xi'an 710032,China)
出处
《河北医学》
CAS
2022年第11期1801-1805,共5页
Hebei Medicine
基金
国家自然科学基金青年项目,(编号:82100218)。
关键词
慢性粒细胞白血病
染色体核型分析
PH染色体
附加染色体异常
Chronic myeloid leukemia(CML)
Chromosome karyotypic analysis
Ph chromosome
Additional chromosome abnormalities
