摘要
目的通过分析网络生物数据探讨丝氨酸/苏氨酸蛋白激酶3(RIPK3)基因在胶质瘤中的表达及分子调控机制。方法应用Gepia2数据库对进行TCGA肿瘤数据和GTEx正常数据联合分析,筛选差异表达基因,进一步对RIPK3基因的调控机制进行分析。荧光实时定量反转录-聚合酶链反应(RT-qPCR)法检测20例胶质瘤组织及正常组织中RIPK3 mRNA、FGD5-AS1、微小RNA(miR)-223-3p的表达,对其关系进行分析。结果Gepia2分析显示,胶质瘤和正常组织中存在7659个差异表达基因。差异表达结果发现RIPK3 mRNA在胶质瘤组织中表达水平(1.862±1.621)显著高于正常组织(0.693±0.801,Z=9.067,P<0.01)。应用STRING数据库分析RIPK3的PPI网络进行富集分析,结果显示RIPK3互相作用的蛋白参与半胱氨酸型内肽酶(cysteine-type endopeptidase)活性、核因子-κB(NF-κB)信号通路等的调控。长非编码RNA FGD5-AS1在胶质瘤中表达水平(6.401±0.683)高于正常组织(5.406±0.796,t=12.697,P<0.01),并与RIPK3呈正相关(rs=0.402,P<0.01);数据库分析显示RIPK3与FGD5-AS1互为内源性竞争RNA(ceRNA),通过miR-223-3p发挥作用。对FGD5-AS1、miR-223-3p、RIPK3 mRNA在胶质瘤及正常组织中表达检测的RT-qPCR结果显示,FGD5-AS1、RIPK3 mRNA在胶质瘤组织中表达水平(1.942±0.695、1.392±0.360)均高于正常组织(0.533±0.208、0.293±0.144,t=8.686、12.676,P<0.01),而miR-223-3p在胶质瘤组织中表达水平(0.843±0.213)低于正常组织(2.334±0.758,t=-8.469,P<0.01)。在胶质瘤组织中RIPK3 mRNA与FGD5-AS1表达呈正相关(rs=0.840,P<0.01),RIPK3 mRNA、FGD5-AS1与miR-223-3p表达呈负相关(rs=-0.914、-0.872,P<0.01)。结论RIPK3在胶质瘤组织中呈高表达,与胶质瘤进展有关。FGD5-AS1可能通过内源性竞争机制通过抑制miR-223-3p而调控RIPK3的表达。
Objective To investigate the expression and molecular regulation mechanism of serine/threonine protein kinase 3(RIPK3)gene in glioma with online biological data.Methods The Gepia2 database was used to conduct joint analysis of TCGA data and GTEx normal data,screen differentially expressed genes,and further analyze the regulatory mechanism of RIPK3 gene.The expression levels of RIPK3 mRNA,FGD5-AS1 and miR-223-3p in 20 glioma tissues and normal tissues were detected by fluorescent real-time quantitative reverse transcription-polymerase chain reaction(RT-qPCR),and their relationship was analyzed.Results Results of Gepia2 analysis revealed that there were 7659 differentially expressed genes in glioma and normal tissues.Differential expression results showed that the expression level of RIPK3 mRNA in glioma tissue(1.862±1.621)was significantly higher than that in normal tissue(0.693±0.801,Z=9.067,P<0.01).STRING database was employed to analyze the PPI network of RIPK3 for enrichment analysis.It was found that RIPK3-interacting proteins were involved in the regulation of cysteine-type endopeptidase(cysteine-type endopeptidase)activity and nuclear factor-κB(NF-κB)signaling pathway.The expression level of long non-coding RNA FGD5-AS1 in gliomas(6.401±0.683)was higher than that in normal tissues(5.406±0.796,t=12.697,P<0.01),and was positively correlated with RIPK3(rs=0.402,P<0.01).It was found that RIPK3 and FGD5-AS1 were endogenous competing RNAs(ceRNA)and played their role through miR-223-3p from database analysis.The RT-qPCR results of FGD5-AS1,miR-223-3p and RIPK3 mRNA expression in glioma and normal tissue showed that the expression level of FGD5-AS1 and RIPK3 mRNA in glioma tissue(1.942±0.695,1.392±0.360)were higher than those in normal tissues(0.533±0.208,0.293±0.144,t=8.686,12.676,P<0.01),while the expression level of miR-223-3p in glioma tissues(0.843±0.213)was lower than that of normal tissue(2.334±0.758,t=-8.469,P<0.01).In glioma tissues,RIPK3 mRNA was positively correlated with FGD5-AS1 expression(
作者
郗艳国
滕伟
赵永辉
程世翔
董晓林
梁赞
王志峰
李国京
Xi Yanguo;Teng Wei;Zhao Yonghui;Cheng Shixiang;Dong Xiaolin;Liang Zan;Wang Zhifeng;Li Guojing(Department of Neurosurgery,Cangzhou Central Hospital,Cangzhou 061000,China;Department of Medicine,Cangzhou Women&Children’s Healthcare Hospital,Cangzhou 061000,China;Department of Research and Development,Xincheng Hospital,Tianjin University,Tianjin 300450,China;Department of Anesthesiology,Cangzhou Central Hospital,Cangzhou 061000,China)
出处
《中华实验外科杂志》
CAS
北大核心
2022年第9期1644-1647,共4页
Chinese Journal of Experimental Surgery
基金
河北省卫生健康委员会医学重点科技研究计划(20220354)。
