摘要
高尿酸血症是多种疾病的诱发因素,而嘌呤代谢紊乱以及尿酸(uric acid,UA)排泄减少是导致高尿酸血症发生的主要原因,因此减少UA的生成以及促进UA的排泄是缓解高尿酸血症的重要途径。茯苓是重要的药食用菌,目前对于茯苓抗高尿酸血症活性的研究甚少。因此,本实验建立了高尿酸血症大鼠模型,评价茯苓粉抗高尿酸血症的功效,同时从UA的产生及排泄方面探讨了茯苓粉降尿酸的机制。结果表明,茯苓粉可高度显著降低高尿酸血症大鼠血清中的UA浓度(P<0.001),同时抑制高尿酸大鼠血清及肝脏中黄嘌呤氧化酶(xanthine oxidase,XOD)活力。此外,茯苓粉可显著改善高尿酸血症大鼠的肾脏功能、肾小球萎缩、肾小管扩张等,同时茯苓粉通过提高肠道中拟杆菌门(Bacteroidetes)、另枝杆菌属(Alistipes)及副杆菌属(Parabacteroides)的相对丰度,降低厚壁菌门(Firmicutes)的相对丰度来调节肠道菌群,推测茯苓粉可通过缓解肾脏损伤及调节肠道菌群来促进机体内UA的排泄,进而达到降低血清UA浓度的效果。本研究结果有利于发掘地方特色食品,同时可为开发茯苓相关产品提供一定的理论参考。
Hyperuricemia is a risk factor for many diseases,and in turn is caused by purine metabolism disorder and the decrease of uric acid(UA)excretion.Therefore,reducing the production of UA and promoting the excretion of UA are significant ways to alleviate hyperuricemia.Although Poria cocos is an important fungus that can be used for both food and medicinal purpose,at present,there are few studies on the anti-hyperuricemic activity of P.cocos.In this context,the current study aimed to evaluate the anti-hyperuricemic effect of P.cocos powder in mice,and elucidate the underlying mechanism from the perspectives of the production and excretion of UA.The results showed that P.cocos powder significantly reduced the serum level of UA(P<0.001)and inhibited the activity of xanthine oxidase(XOD)in the serum and liver of hyperuricemic rats.In addition,P.cocos powder remarkably improved renal function,glomerular atrophy,and renal tubule dilatation in hyperuricemic rats.Meanwhile,P.cocos powder significantly increased the relative abundance of Bacteroidetes,Alistipes and Parabacteroides,and significantly reduce the relative abundance of Firmicutes in the intestinal tract.These results speculated that P.cocos powder could promote the excretion of UA to reduce serum UA concentration by alleviating kidney damage and regulating the intestinal flora.These findings are conducive to the discovery of local specialties,and also provide some theoretical reference for the development of P.cocos related products.
作者
王琨
吴珊珊
黎攀
肖南
杜冰
WANG Kun;WU Shanshan;LI Pan;XIAO Nan;DU Bing(College of Food Science,South China Agricultural University,Guangzhou 510642,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第21期171-179,共9页
Food Science
基金
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-21)
广东省重点领域研发计划项目(2020B020226008)
广东省自然科学基金面上项目(2020A1515011268)
云南省杜冰专家工作站项目(202005AF150071)。
关键词
茯苓
高尿酸血症
肾损伤
肠道菌群
Poria cocos
hyperuricemia
renal damage
gut microbiota
