摘要
为了研究高氧胁迫下西兰花抗氧化系统的响应机制,本实验基于同位素相对与绝对定量蛋白质组学结合生理生化指标分析,探究不同O_(2)体积分数(5%O_(2)+5%CO_(2)、20%O_(2)+5%CO_(2)和40%O_(2)+5%CO_(2))下西兰花抗氧化系统及相关蛋白表达的变化。基因本体论注释、同源蛋白簇功能分析、京都基因与基因组百科全书通路分析结果表明,本研究共鉴定和筛选出76个与抗氧化系统相关的差异表达蛋白,分别涉及抗坏血酸-谷胱甘肽循环、硫代葡萄糖苷降解、多酚及类黄酮等抗氧化物质代谢、抗氧化酶代谢等途径。蛋白质组学分析结果表明,相比于5%O_(2)+5%CO_(2)处理,40%O_(2)+5%CO_(2)处理抑制了1种抗坏血酸过氧化物酶活力和3种谷胱甘肽过氧化物酶活力,抑制了西兰花的抗坏血酸-谷胱甘肽循环;同时40%O_(2)+5%CO_(2)处理明显增加了16种黑芥子酶活力,促进了硫代葡萄糖苷降解。此外,40%O_(2)+5%CO_(2)处理莽草酸脱氢酶的表达差异倍数仅为5%O_(2)+5%CO_(2)处理的0.77倍,抑制了莽草酸合成和多酚及类黄酮积累。本研究揭示了高氧导致西兰花黄化衰老的原因,可为西兰花保鲜技术的开发及高氧胁迫的缓解提供理论依据。
In order to explore the molecular mechanism of the response of the antioxidant system in broccoli heads to high O_(2) stress,the changes in the antioxidant system and related protein expression in broccoli heads under different O_(2) concentrations(5%O_(2)+5%CO_(2),20%O_(2)+5%CO_(2) and 40%O_(2)+5%CO_(2))were investigated by physiological and biochemical analysis and isobaric tags for relative and absolute quantitation(iTRAQ)proteomics.The gene ontology(GO)annotation,the clusters of orthologous groups of proteins(COG)analysis and the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that a total of 76 differentially expressed proteins related to the antioxidant system were identified in this study,which were involved in the ascorbate-glutathione cycle,glucosinolate degradation,and the metabolism of polyphenols,flavonoids and antioxidant enzymes.The proteomic analysis showed that the 40%O_(2)+5%CO_(2) treatment significantly inhibited the activity of an ascorbate peroxidase and three glutathione peroxidases,thereby inhibiting the ascorbate-glutathione cycle in broccoli compared with the 5%O_(2)+5%CO_(2) treatment.Meanwhile,the 40%O_(2)+5%CO_(2) treatment significantly increased the activities of 16 myrosinases and promoted the degradation of glucosinolate.In addition,the expression of shikimate dehydrogenase in the 40%O_(2)+5%CO_(2) treatment group decreased by 23%compared with that in the 5%O_(2)+5%CO_(2) treatment group,inhibiting shikimic acid synthesis and the accumulation of polyphenols and flavonoids.This study has revealed the cause of rapid yellowing and senescence in broccoli heads under high O_(2) stress,which provides a theoretical basis for the development of new broccoli preservation technology and the remission of high O_(2) stress.
作者
张玉笑
陈颖
郭衍银
马阳历
杨梅
付瑞青
孙玉芃
ZHANG Yuxiao;CHEN Ying;GUO Yanyin;MA Yangli;YANG Mei;FU Ruiqing;SUN Yupeng(College of Agricultural Engineering and Food Science,Shandong University of Technology,Zibo 255049,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第21期34-42,共9页
Food Science
基金
国家自然科学基金面上项目(31671900)。
