摘要
随地球环境污染,气候变化问题愈发严重,植物生长过程中面临的极端环境风险也随之增加,植物所受环境胁迫中缺铁胁迫广泛存在。文章以拟南芥基因组DNA为模板,聚合酶链式反应(polymerase chain reaction,PCR)扩增克隆拟南芥MYC1基因启动子区域,经过酶切和连接,与GUS(β-葡萄糖苷酸酶)表达载体构建融合表达载体ProMYC1-GUS。通过浸花法侵染野生型拟南芥植株获得转基因材料,并对ProMYC1-GUS转基因材料进行缺铁诱导GUS组织染色,证明植物MYC1基因在缺铁胁迫下表达受到抑制。
With the global environmental pollution,the problem of climate change is becoming more and more serious,and the extreme environmental risks faced by plants during the growth process have also increased.Among the environmental stresses experienced by plants,iron deficiency stress is widespread and harmful.In this study,Arabidopsis thaliana genomic DNA was used as a template,and the promoter region of Arabidopsis MYC1 gene was cloned by polymerase chain reaction(PCR).After double restriction enzyme digestion and ligation,the fusion expression vector ProMYC1-GUS was constructed with the GUS(β-glucuronidase)expression vector.Transgenic materials were obtained by infecting wild-type Arabidopsis plants by the method of floral-dip,and GUS staining of ProMYC1-GUS transgenic materials induced by iron deficiency showed that the expression of MYC1 genes in plants was inhibited by iron deficiency.
作者
耿庆鎏
宋慧
胡敏
曹树青
GENG Qingliu;SONG Hui;HU Min;CAO Shuqing(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China)
出处
《合肥工业大学学报（自然科学版）》
CAS
北大核心
2022年第11期1580-1584,共5页
Journal of Hefei University of Technology：Natural Science
基金
国家自然科学基金资助项目(2307020331)。
关键词
环境胁迫
缺铁胁迫
MYC1基因
载体构建
GUS染色
environmental stress
iron deficiency stress
MYC1 gene
vector construction
GUS staining
