摘要
以π/2相移间隔的结构光照明显微图像为输入,通过图像差分、数学推导及简化构建一个数学模型,在空间域直接解析得到超分辨图像。理论验证该模型可将传统显微的横向分辨率提高1倍,并可减少离焦背景干扰。在基于数字微镜器件的结构光照明显微系统中,对荧光微球及牛肺动脉内皮细胞进行空间域超分辨重建实验,验证了算法的有效性。获得的超分辨效果与频域重建法相近,但图像重建速度快约5倍。该研究有助于扩展结构光照明显微的应用范围,对活细胞和组织的长时间在体监测具有良好的应用潜力。
The spatial resolution of traditional optical microscopy imaging technology is limited by the optical diffraction limit,which can only reach the order of half wavelength of the incident wavelength,which greatly limits the application scope of optical microscopy technology.Among the mainstream optical super-resolution microscopy techniques,Structured Illumination Microscopy(SIM)is an attractive choice for fast super-resolution microscopy due to its fast imaging speed,low phototoxicity,and no additional complex sample preparation process.SIM technology applies periodic sinusoidal fringe structured light with a spatial frequency close to the diffraction limit to the sample.The high-frequency information that could not pass through the sample is now converted into a low-frequency “Moire fringe”,which couples the highfrequency information of the sample beyond the diffraction limit to the imaging.The frequency region detectable by the system can theoretically double the lateral resolution of the microscope.The SIM image reconstruction algorithm is the key to determine the final super-resolution image quality.Traditional frequency-domain image reconstruction algorithms need to estimate the initial phase,spatial frequency and other parameters of the structured light field.It is also necessary to perform multiple Fourier transforms between the spatial domain and the frequency domain.The operation speed is slow affecting its application in real-time dynamic imaging and other fields.The proposed spatial-domain super-resolution imaging algorithms are currently limited to solving the 2π/3 phase shift for structured light super-resolution reconstruction.In the traditional structured illumination microscopy based on the projection of the digital micromirror device,the structured fringes with a period of 4 pixels need to adopt a phase shift interval of π/2.The spatial domain reconstruction algorithm under this application has not been reported yet.Here,a superresolution image reconstruction for SIM in the Spatial domain is
作者
黄彩虹
朱星星
郭威
易定容
金福江
黄磊
HUANG Caihong;ZHU Xingxing;GUO Wei;YI Dingrong;JIN Fujiang;HUANG Lei(College of Mechanical Engineering and Automation,Huaqiao University,Fujian 361021,China;College of Information Science and Engineering,Huaqiao University,Fujian 361021,China;School of Life Science,Xiamen University,Fujian 361102,China)
出处
《光子学报》
EI
CAS
CSCD
北大核心
2022年第11期222-232,共11页
Acta Photonica Sinica
基金
福建省自然科学基金重点项目(No.2020J02005)
福建省自然科学基金面上项目(No.2021J01293)。
关键词
结构光照明
显微镜
超分辨率
图像重建
空间域
Structured illumination
Microscopy
Super-resolution
Image reconstruction
Spatial domain
