摘要
细菌微室(BMCs)是一种原核生物细胞器,由半渗透性的蛋白质外壳和被封装的酶构成,其结构保守而功能非常多样化。蛋白质外壳可以将特定的代谢过程与细胞质环境分隔开来,酶和代谢中间体的区室化可以增加代谢反应的通量并保护细胞免受有毒中间体的影响。因此,细菌微室提高了细菌的代谢多样性与环境适应力。同时,在以细菌微室为代谢元件、外壳蛋白改造、人工构建新型细菌微室等多个方面都取得了一定的研究进展。细菌微室的上述特性表明其在合成生物学中具有巨大的应用潜力,以细菌微室为基础的合成生物学改造,有望推动绿色生物制造快速发展。
Bacterial microcompartments(BMCs)were organelles composed of proteins including a semi⁃permeable shell and encapsulated enzymes in prokaryotes cells.Their structure was conservative and the functions were very diverse.The protein shell could separate specific metabolic steps from the cytoplasmic environment,and the compartmentalization of enzymes and metabolic intermediates could increase the flux of metabolic reactions and protect cells from toxic intermediates.Therefore,BMCs improved the metabolic diversity and environmental adaptability of bacteria.Research progresses in BMCs were summarized,such as using BMCs as metabolic elements,shell protein modification,artificial construction of new BMCs,and many aspects had achieved considerable progress.The characteristics of BMCs determined that there was a huge application potential in synthetic biology,and the fast growing of green biomanufacturing was promoted by the synthetic biology reformation of BMCs.
作者
张俊哲
张全
刘自勇
马小清
刘立成
李福利
ZHANG Junzhe;ZHANG Quan;LIU Ziyong;MA Xiaoqing;LIU Licheng;LI Fuli(Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China;Sinopec Dalian(Fushun)Research Institute of Petroleum and Petrochemicals,Dalian 116045,China;Shandong Energy Institute,Qingdao 266101,China)
出处
《南京工业大学学报（自然科学版）》
CAS
北大核心
2022年第5期490-499,共10页
Journal of Nanjing Tech University(Natural Science Edition)
基金
国家自然科学基金联合基金重点项目(U21B2099)
山东能源研究院专项基金(SEI S202104)
中国科学院洁净能源创新研究院合作基金(DNL202013)。
关键词
细菌微室
合成生物学
代谢工程
原核生物
细胞器
bacterial microcompartments(BMCs)
synthetic biology
metabolic engineering
prokaryotes
organelles
