摘要
本研究获得了薰衣草转录因子MYB基因,并探究了该基因在薰衣草不同组织、花器官不同时期的表达特性和转录激活活性。采用RACE和RT-PCR技术克隆了薰衣草(Lavandula angustifolia Mill.)LaMYB1转录因子,该基因全长729 bp,编码242个氨基酸,蛋白分子量为27.60 kDa,等电点为9.01。该基因与白桦BpMYB21基因亲缘关系较近。薰衣草LaMYB1基因在‘杂花’和‘法国蓝’两个品系不同组织和花器官不同时期的表达规律具有相似性,该基因在雄蕊的表达量最高,在衰败期的表达量最高。α-蒎烯、乙酸薰衣草酯、氧化石竹烯、莰烯和柠檬烯在雄蕊中的含量最高,这些萜类物质在两个品系不同组织中的含量变化规律与LaMYB1基因在不同组织中的相对表达量变化规律一致;LaMYB1基因在‘法国蓝’薰衣草中的相对表达量最高,右旋樟脑和柠檬烯的含量在‘法国蓝’薰衣草衰败期最高。薰衣草LaMYB1基因具有转录激活活性。
In this study,MYB gene of lavender transcription factor was obtained,and the expression characteristics and transcriptional activation activity of MYB gene in different tissues and flower organs of lavender were explored.The LaMYB1 transcription factor of Lavandula angustifolia Mill.was cloned by RACE and RT-PCR.The total length of LaMYB1 gene was 729 bp,encoding 242 amino acids,with molecular weight of 27.60 kDa and isoelectric point of 9.01.The gene was closely related to BpMYB21 gene of Betula platyphylla.The expression patterns of LaMYB1 gene in different tissues and flower organs of‘ZaHua’and‘French blue’were similar,and the expression level of LaMYB1 gene was the highest in stamen and decayed stage.The contents ofα-pinene,lavender acetate,caryophyllene oxide,camphene and limonene were the highest in stamens.The contents of these terpenes in different tissues of the two strains were consistent with the relative expression of LaMYB1 gene in different tissues.The relative expression level of LaMYB1 gene was the highest in‘French blue’lavender,and the contents of dexcamphor and limonene were the highest in‘French blue’lavender during decline stage.LaMYB1 gene of lavender has transcriptional activation activity.
作者
周迪
李田田
龙遗磊
严中建
买尔孜亚古丽·阿不来克木
苏秀娟
ZHOU Di;LI Tian-tian;LONG Yi-lei;YAN Zhong-jian;Maierziyaguli Abulaikemu;SU Xiu-juan(Key Laboratory of Crop Genetic Improvement and Germplasm Enhancement,College of Agronomy,Xinjiang Agricultural University,Urumqi 830000,China)
出处
《新疆农业大学学报》
CAS
2022年第2期94-100,共7页
Journal of Xinjiang Agricultural University
基金
国家自然科学基金项目(31960452)
新疆维吾尔自治区“天山青年计划”优秀青年科技培养项目(2020Q016)
新疆维吾尔自治区区域协同创新专项(科技援疆计划)项目(2020E0221)。
关键词
薰衣草
MYB转录因子
萜类物质
表达分析
转录激活活性
lavender
MYB transcription factor
terpenes
expression analysis
transcriptional activation activity
