摘要
通过比较患者/健康人代谢物中挥发性有机物(volatile organic compounds,简称VOCs),可以获得相关的生物标志物,并有望用于疾病的无创筛查.但消化系统健康器官本身是否具有相互差异性的VOCs并不清楚,为此,笔者以大鼠为模型动物,开展了消化系统主要器官VOCs的检测分析,以获得差异性VOCs特征.利用顶空固相微萃取气相色谱质谱(solid phase microextraction-gas chromatography-mass spectrometry,简称SPME-GC-MS)技术,对8只大鼠消化系统的肝脏、胃、小肠、盲肠、大肠的离体组织中VOCs进行检测,在色谱峰的非靶向分析中,通过与其他器官组织的比较,借助Mann-Whitney U检验(p<0.05)和逐步判别分析,发现2种器官组织存在差异性VOCs,即肝脏中的丙酮、正戊醇以及盲肠中的苯酚、丙酸、丙基丙二酸、苯并噻唑.在此基础上,对器官差异性VOCs的可能代谢途径以及相关的标志物进行了讨论.该研究首次报告了大鼠消化系统主要器官差异性VOCs,对人体代谢VOCs相关研究将具有参考意义.
By the comparative study of volatile organic compounds(VOCs) in the metabolites between patients and healthy people, the relevant biomarkers can be obtained, which are expected to be used for noninvasive screening of diseases. However, it is not clear whether the healthy organs of the digestive system themselves have differential VOCs. Therefore, this study used rat as model animal to perform a preliminary study on the analysis of VOCs in the main organs of the digestive system, so as to obtain the characteristics of differential VOCs. Using headspace solid phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS), the VOCs from ex vivo tissues of liver, stomach, small intestine, cecum and large intestine were detected for eight rats. In the untargeted analysis of chromatographic peaks, by comparing with other organ tissues and using Mann-Whitney U test(p <0.05) as well as stepwise discriminant analysis, it was found that two organ tissues had differential VOCs, namely, acetone and 1-pentanol in the liver and phenol, propionic acid, 2-propylmalonic acid and benzothiazole in the cecum. On this basis, the possible metabolic pathways of these organ differential VOCs and the associated biomarkers were discussed. This study reported the differential VOCs for the main organs in the rat digestive system for the first time, which would have reference significance for the related study on human metabolic VOCs.
作者
刘玥
柯立
陆燕
张兵
邹雪
夏磊
李爱悦
刘亚伟
黄超群
沈成银
储焰南
LIU Yue;KE Li;LU Yan;ZHANG Bing;ZOU Xue;XIA Lei;LI Aiyue;LIU Yawei;HUANG Chaoqun;SHEN Chengyin;CHU Yannan(Institutes of Physical Science and Information Technology,Anhui University,Hefei 230601,China;Anhui Province Key Laboratory of Medical Physics and Technology,Institute of Health and Medical Technology,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China;Department of Thoracic Surgery,The First Affiliated Hospital of University of Science and Technology of China,Hefei 230001,China;Hefei Cancer Hospital,Chinese Academy of Sciences,Hefei 230031,China)
出处
《安徽大学学报（自然科学版）》
CAS
北大核心
2022年第6期76-83,共8页
Journal of Anhui University(Natural Science Edition)
基金
国家自然科学基金资助项目(21876176,21777163,22076190)
中科院合肥研究院院长基金资助项目(YZJJZX202009)
中科院青促会资助项目(2019432)
安徽医科大学第二附属医院-中科院合肥研究院医学物理中心联合基金资助项目(LHJJ2020006)
安徽省科技厅重点研发计划(科技强警)项目(202104d07020003)。
