摘要
2020—2021年湖北烟区爆发新型烟草叶部病害,本研究对病原菌进行分离培养、形态观察、镜检菌丝和科赫法则验证,并提取30个菌株的DNA使用通用引物ITS1/ITS4进行rDNA-ITS序列的PCR扩增,及MEGA-X软件进行基因序列比对及系统发育树构建,同时以Rs1F2/Rs2R1为引物,进行30个菌株的r DNA-ITS基因的PCR专化性鉴定。结果表明,该病害病原菌为立枯丝核菌(Rhizoctonia solani Kühn),30个菌株序列都隶属于R. solani AG-3融合群且均具有AG-3融合群所特有的504 bp片段。因此,确定该病害为烟草靶斑病。这是首次从湖北省烟草产区分离鉴定到该病菌。
In 2020 and 2021, a new type of tobacco leaf disease broke out in the main tobacco producing areas of Hubei Province.The pathogen was analyzed through isolation and morphological observation, microscopic examination of hyphae and verification of Koch’s postulates, extraction of the DNA of 30 strains and amplification of the rDNA-ITS sequence by PCR with universal primers ITS1/ITS4. The MEGA-X software was used for gene sequence alignment and phylogenetic tree construction, and the PCR specificity of rDNA-ITS genes of 30 strains was identified with Rs1F2/Rs2R1 as primers. The results showed that the pathogen was identified as Rhizoctonia solani Kühn, all 30 strains belonged to the R. solani AG-3 anastomosis group and with a 504 bp fragment unique to AG-3 anastomosis group. Therefore, the disease was identified as tobacco target spot. This is the first time that the pathogen was isolated from tobacco producing areas in Hubei Province.
作者
邱梦娟
黎妍妍
徐婷婷
刘涵斐
吴辰飞
郑露
黄俊斌
李锡宏
QIU Mengjuan;LI Yanyan;XU Tingting;LIU Hanfei;WU Chenfei;ZHENG Lu;HUANG Junbin;LI Xihong(Key Laboratory of Plant Pathology of Hubei Province,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Tobacco Research Institute of Hubei Province,Wuhan 430030,China)
出处
《中国烟草科学》
CSCD
北大核心
2022年第5期50-55,共6页
Chinese Tobacco Science
基金
中国烟草总公司湖北省公司科技项目(027Y2021-004
027Y2022-020)。
关键词
烟草靶斑病
立枯丝核菌
病原鉴定
菌丝融合群
tobacco target spot
Rhizoctonia solani
pathogen identification
anastomosis group
