摘要
为了研究不同氮效率玉米品种伟科518(氮高效品种,WK518)、农大108(氮低效品种,ND108)在氮减施条件下的差异表达基因(Differently expressed genes,DEG)及其功能,在不同氮条件下[正常氮(225 kg/hm^(2),HN)、低氮(0 kg/hm^(2),LN)],对灌浆中期WK518和ND108穗位叶进行高通量转录组测序,筛选DEG,进行GO和KEGG富集分析,并对差异表达的转录因子进行分析。结果表明,低氮条件下,在WK518与ND108对比组中共检测到2065个上调表达基因、2319个下调表达基因。正常氮条件下,在WK518与ND108对比组中共检测到2368个上调表达基因、3780个下调表达基因。WK518在不同氮条件下共检测到1009个上调表达基因、2268个下调表达基因。ND108在不同氮条件下共检测到364个上调表达基因、510个下调表达基因。低氮条件下,WK518与ND108中的DEG主要富集于尿卟啉-ⅢC-甲基转移酶活性、甘露糖-6-磷酸异构酶活性、氧化-还原过程、线粒体组织、核染色质等GO条目,氨基糖及核苷酸糖新陈代谢,苯丙氨酸新陈代谢,类单萜生物合成,甘氨酸、丝氨酸及苏氨酸新陈代谢、碱基剪切修复等KEGG代谢通路。正常氮条件下,WK518与ND108中的DEG主要富集于气孔关闭、跨膜受体丝氨酸/苏氨酸蛋白激酶活性、叶绿体基质、类囊体、叶绿体被膜等GO条目,光合作用生物体中碳固定、氨基糖及核苷酸糖新陈代谢、糖酵解/糖异生、丙氨酸新陈代谢、光合作用-天线蛋白等KEGG代谢通路。WK518在不同氮条件下的DEG主要富集于几丁质反应、蛋白质磷酸化、生物膜、吲哚硫代葡萄糖苷代谢过程、肌醇半乳糖苷-蔗糖半乳糖基转移酶等GO条目,光合作用生物体中碳固定、植物激素信号传导、内质网内蛋白质加工过程、植物-病原体相互作用、植物MAPK信号通路等KEGG代谢通路。ND108在不同氮条件下的DEG主要富集于干旱胁迫响应、毒素分解代谢过程、几丁质酶活性、�
In order to explore the differentially expressed genes(DEGs)in Weike 518(WK518)with high nitrogen efficiency and Nongda108(ND108)with low nitrogen efficiency under nitrogen reduction condition and their functions,the ear-leaf samples were collected from WK518 and ND108 at mid filling stage under normal nitrogen fertilizer application rate(225 kg/ha nitrogen,HN)and low nitrogen fertilizer application rate(0 kg/hanitrogen,LN),and used for high-throughput RNA sequencing.Then the GO term and KEGG metabolic pathway of DEGs were analyzed from different compared groups,and the differentially expressed transcription factor families were analyzed.The results showed that 2065up-regulated DEGs and 2319 down-regulated DEGs were detected between WK518 and ND108 under LN condition,2368 up-regulated DEGs and 3780 down-regulated DEGs were detected under HN condition;1009 up-regulated DEGs and 2268 down-regulated DEGs were detected in WK518 under different nitrogen application rates,and 364 up-regulated DEGs and 510 down-regulated DEGs were detected in ND108 under different nitrogen application rates.Under LN condition,DEGs between WK518 and ND108 were mainly enriched in uroporphyrin-ⅢC-methyltransferase activity,mannose-6-phosphate isomerase activity,oxidation-reduction process,mitochondrion organization,nuclear chromatin and other GO terms,and amino sugar and nucleotide sugar metabolism,phenylalanine metabolism,monoterpenoid biosynthesis,glycine/serine and threonine metabolism,base excision repair and other KEGG pathways.Under HN condition,DEGs between WK518 and ND108 were mainly enriched in stomatal closure,transmembrane receptor protein serine/threonine kinase activity,chloroplast stroma,thylakoid,chloroplast envelope and other GO terms,and carbon fixation in photosynthetic organisms,amino sugar and nucleotide sugar metabolism,glycolysis/gluconeogenesis,β-alanine metabolism,photosynthesis-antenna proteins and other KEGG pathways.DEGs in WK518 were mainly enriched in response to chitin,protein phosphorylation,membrane,indol
作者
李川
张盼盼
张美微
牛军
赵霞
何冠华
乔江方
LI Chuan;ZHANG Panpan;ZHANG Meiwei;NIU Jun;ZHAO Xia;HE Guanhua;QIAO Jiangfang(Cereal Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Agricultural Science Research Institute of Baofeng County,Baofeng 467400,China)
出处
《河南农业科学》
北大核心
2022年第9期10-24,共15页
Journal of Henan Agricultural Sciences
基金
河南省农业科学院优秀青年科技基金项目(2022YQ03)
河南省自然科学基金青年科学基金项目(202300410526)。
关键词
玉米
氮肥减施
氮效率
高通量转录组测序
差异表达基因
Maize
Nitrogen reduction
Nitrogen efficiency
High-throughput RNA sequencing
Differentially expressed gene(DEG)
