摘要
为研究牛樟芝胞外多糖的抗氧化活性及其在胃肠系统中的消化特性,采用原位萃取发酵技术进行牛樟芝的液态发酵,并考察发酵萃取剂对多糖产量的影响;通过·O^(-)_(2)、·OH以及DPPH自由基清除率的测定评估胞外多糖的抗氧化性;构建体外模拟胃肠液和人源粪便的消化模型分析牛樟芝胞外多糖的体外消化特性。结果显示,油酸萃取发酵能够显著提高牛樟芝胞外多糖产量(236.42 mg/L)及抗氧化活性;利用简单醇沉就能够获得单一胞外多糖组分AC-2,其分子质量为23.07 kDa,主要由岩藻糖、半乳糖、葡萄糖和甘露糖组成,物质的量比为0.09∶1∶0.49∶0.27。此外,AC-2能够稳定通过胃肠液系统而不被消化降解并最终被肠道微生物利用;在人源粪便体外发酵过程中,AC-2显著降低肠道pH并提高短链脂肪酸(short chain fatty acids,SCFAs)含量,表明AC-2能够有效改善肠道环境。综上,AC-2作为一种小分子胞外多糖,能够稳定通过胃肠液系统,促进肠道微生物合成SCFAs,具有调节肠道健康的潜在活性。
This study aimed to investigate the antioxidant activity and digestion properties in gastrointestinal system of the exopolysaccharides from Antrodia camphorata.In situ extraction fermentation was applied in submerged fermentation of A.camphorata and the effect of extractants on the yield of exopolysaccharides was investigated.To evaluate the antioxidant activity of the exopolysaccharides,the superoxide anion,hydroxyl radical,and DPPH radical scavenging rate were texted.Furthermore,the model of digestion and fecal fermentation in vitro was used to analyze its digestion properties.The results showed that oleic acid extraction fermentation could significantly increase the exopolysaccharide yield(236.42 mg/mL) and antioxidant activity.The exopolysaccharide AC-2 was obtained by alcohol precipitation with molecular weight of 23.07 kDa.It was mainly composed of fucose,galactose,glucose,and mannose,with the molar ratio of 0.09∶1∶0.49∶0.27.Moreover,AC-2 could transit through the gastrointestinal tract without dramatic degradation and then utilized by gut microbiota.During the fermentation process by human feces,AC-2 exhibited a high capability to decrease pH and increase the content of short-chain fatty acids(SCFAs),which indicated that AC-2 could improve the intestinal environment.In summary,AC-2 is a small molecule polysaccharide that can stably through the gastrointestinal and promote the intestinal microbes to synthesize SCFAs,indicating that AC-2 has the potential activity in regulating intestinal health.
作者
梁丽红
李辉
关莹杰
李哲远
杨晓辰
张汇
王光强
熊智强
艾连中
夏永军
LIANG Lihong;LI Hui;GUAN Yingjie;LI Zheyuan;YANG Xiaochen;ZHANG Hui;WANG Guangqiang;XIONG Zhiqiang;AI Lianzhong;XIA Yongjun(School of Health Science and Engineering,Shanghai Engineering Research Center of Food Microbiology,University of Shanghai for Science and Technology,Shanghai 200093,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2022年第21期82-89,共8页
Food and Fermentation Industries
基金
国家自然科学基金面上项目(31871757)
上海食品微生物工程技术研究中心项目(19DZ2281100)
上海理工大学科研课程项目(19900010)。
关键词
牛樟芝
胞外多糖
抗氧化
体外模拟消化
粪便发酵
Antrodia camphorata
exopolysaccharides
antioxidant
simulated digestion in vitro
fecal fermentation
