摘要
目的 分析体液游离细胞环状RNA LRP6(circ-LRP6)对食管鳞状细胞癌(ESCC)诊断和预后的评估效能。方法 选取2017年8月至2020年2月56例在我院接受根治性切除手术的ESCC患者和50例健康对照组人群的血清、尿液、唾液样本。采用实时荧光定量PCR(q PCR)检测游离细胞circ-LRP6表达。采用受试者工作特征(ROC)曲线评价体液游离细胞circ-LRP6诊断ESCC的效能。分析circ-LRP6表达与患者临床特征和预后的关系。结果 ESCC患者的血清[4.90(2.27,9.91) vs. 0.64(0.24,1.04)]、尿液[2.31(0.89,5.26) vs.0.43(0.16,1.07)]、唾液[0.78(0.14,2.83) vs. 0.08(0.03,0.26)]游离细胞circ-LRP6均显著高于健康对照组(P<0.001)。ROC曲线结果显示,血清、尿液、唾液游离细胞circ-LRP6诊断ESCC的曲线下面积分别为0.897、0.803、0.793。TNM分期和T分期与3种体液游离细胞circ-LRP6表达均有关(P<0.05)。血清、尿液和唾液中游离细胞circ-LRP6水平检测ESCC的灵敏度分别为80.6%、72.5%和80.4%,联合灵敏度为97.1%,高于常规肿瘤标志物的诊断敏感性(P<0.05)。Kaplan-Meier生存曲线结果显示,血清、尿液、唾液游离细胞circ-LRP6高表达者的3年总生存率均较低表达者低(P<0.05)。多因素Cox风险比例回归模型结果显示,SCC-Ag及血清、尿液、唾液游离细胞circ-LRP6表达均为影响ESCC患者OS的独立预后因素(P<0.05)。结论 血清、尿液、唾液游离细胞circ-LRP6表达均对可切除ESCC有良好诊断及预测预后效能。
Objective To evaluate the diagnostic and prognostic value of body fluid free cell circular RNA LRP6(circ-LRP6)in esophageal squamous cell carcinoma(ESCC).Methods Serum,urine and saliva samples were collected from 56 ESCC patients who underwent radical resection in our hospital and 50 healthy controls from August 2017 to February 2020.The expression of circ-LRP6 in free cells was detected by real-time PCR(qPCR).The receiver operating characteristic(ROC)curve was used to evaluate the efficacy of circ-LRP6 in the diagnosis of ESCC.The relationship between circ-LRP6 expression and clinical characteristics and prognosis of patients was analyzed.Results In ESCC patients serum[4.90(2.27,9.91)vs.0.64(0.24,1.04)],urine[2.31(0.89,5.26)vs.0.43(0.16,1.07)],saliva[0.78(0.14,2.83)vs.0.08(0.03,)]0.26)]circ-LRP6 of free cells was significantly higher than that of healthy control group(P<0.001).ROC curve results showed that the areas under the curve of serum,urine and saliva free cells circ-LRP6 in the diagnosis of ESCC were 0.897,0.803 and 0.793,respectively.TNM stage and T stage were correlated with the expression of circ-LRP6 in three body fluid free cells(P<0.05).The sensitivity of circ-LRP6 in serum,urine and saliva for the detection of ESCC was 80.6%,72.5%and 80.4%,respectively,and the combined sensitivity was 97.1%,which was higher than that of conventional tumor markers(P<0.05).Kaplan-meier survival curve results showed that the 3-year overall survival rate of patients with high expression of circ-LRP6 in serum,urine and saliva free cells was lower than that of patients with low expression of circ-LRP6(P<0.05).Multivariate Cox proportional hazard regression model showed that SCC-AG and circ-LRP6 expression in serum,urine and saliva free cells were independent prognostic factors affecting OS in ESCC patients(P<0.05).Conclusion The expression of circ-LRP6 in serum,urine and saliva free cells has good diagnostic and prognostic efficacy for resectable ESCC.
作者
岳亚玲
王继云
朱佳乐
YUE Yaling;WANG Jiyun;ZHU Jiale(Department of Oncology,West District,Handan Central Hospital,Hebei Province,Handan 056001,China)
出处
《临床肿瘤学杂志》
CAS
2022年第10期878-884,共7页
Chinese Clinical Oncology
