摘要
葡萄糖代谢是机体能量代谢的核心,在各个组织器官发挥能量供应及代谢调控作用,胰岛素是机体内唯一的降糖激素。胰岛功能及胰岛素敏感性是影响葡萄糖代谢的关键环节。葡萄糖钳夹技术是评估胰岛素敏感性及胰岛功能的金标准,主要类别包括高胰岛素正糖钳夹、高糖钳夹、高胰岛素低糖钳夹等类型。在小鼠葡萄糖钳夹实验中根据是否麻醉分为麻醉和清醒状态钳夹。本文重点阐述了小鼠葡萄糖钳夹技术方法的建立及操作的具体流程,包括器械耗材的准备、手术操作、钳夹过程及注意事项,旨在为实验室搭建各类小鼠葡萄糖钳夹技术平台提供参考。
Glucose metabolism plays a central role in energy supply and metabolism regulation in various tissues and organs.Besides,insulin is the sole hormone lowering blood glucose in the body,and islet function and insulin sensitivity are the key steps modulating glucose metabolism.Since the development of glucose clamp technology,it has been recognized as the gold standard for evaluating insulin metabolism.The main categories include hyperinsulinemia-euglycemia clamp,hyperglycemia clamp,and hyperinsulinemia-hypoglycemia clamp.These can be done on either anesthetized mice or conscious and unrestricted mice.This protocol focuses on the establishment and operation of the mouse glucose clamp technique,including preparation of instrument consumables,surgical operations,clamping procedures,and precautions,serving as reference and guidance.
作者
龚颖芸
付真真
周红文
Yingyun Gong;Zhenzhen Fu;Hongwen Zhou(Department of Endocrinology and Metabolism,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)
出处
《遗传》
CAS
CSCD
北大核心
2022年第10期967-974,共8页
Hereditas（Beijing)
基金
国家自然科学基金项目(编号:31900832,82170882)
江苏省科技厅基础研究计划(编号:BK20191074)资助。
关键词
高胰岛素正糖钳夹
高糖钳夹
高胰岛素低糖钳夹
胰岛素敏感性
胰岛功能
hyperinsulinemia-euglycemia clamp
hyperglycemia clamp
hyperinsulinemia-hypoglycemia clamp
insulin sensitivity
beta-cell function
