摘要
背景:Bio-oss骨粉、奥邦骨修复材料均为常用的骨修复材料,但其对兔股骨缺损后修复效果的系统性评价鲜有报道。目的:利用同步辐射显微断层成像技术和组织病理学染色相结合,系统评价Bio-oss骨粉和奥邦骨修复材料修复骨缺损的效果。方法:采用随机数表法将32只大白兔随机分为对照组(n=12)与实验组(n=20),均于一侧股骨体部外侧面建立2个大小为4 mm(洞空直径)×6 mm(洞孔深度)的洞穿型骨膜骨质缺损,对照组填充生理盐水,实验组其中一个骨缺损部位植入Bio-oss骨粉,另一骨缺损部位植入奥邦骨修复材料。术后8周,通过大体观察、影像学、组织病理学检测评价2种材料修复材料的修复效果。结果与结论:①大体观:术后8周,实验组骨损伤修复区伤口愈合良好,未见感染、化脓等症状,动物行动能力显著优于对照组;②影像学检查:术后8周同步辐射显微断层成像,Bio-oss骨粉植入后骨粉周围存在大量的新生骨组织,骨缺损空腔较大,未被新生骨组织完全填充;奥邦修复材料植入后可见骨缺损腔内围绕修复材料形成了大量的新生骨组织;三维重建显示,两种骨填充材料都能与局部骨创面组织接触,促进周围成骨分化成新生骨组织和血管形成,达到骨修复效果,其中奥邦骨修复材料周围新生骨与周围骨组织连接更紧密。256 iCT扫描显示,奥邦骨修复材料侧术后8周CT值高于Bio-oss骨粉侧(P<0.05);③组织病理学:术后8周苏木精-伊红染色显示,Bio-oss骨粉侧骨粉周围可见新生骨组织,奥邦骨修复材料侧材料周围可见由骨基质形成的骨组织,新生骨组织完整、连贯,已有部分新生骨侵入到奥邦骨粉材料内部;④结果表明,奥邦骨修复材料能促进新生骨的生成和损伤修复;同步辐射显微断层成像技术与传统的苏木精-伊红染色相呼应,是评价骨粉修复成效的良好方法。
BACKGROUND:Bio-oss bone powder and Aobang repair materials are commonly used bone repair materials,but there are few reports on their systematic evaluation of the repair effect of rabbit femoral defect.OBJECTIVE:To systematically evaluate the repair effects of Bio-oss bone powder and Aobang repair materials on femoral defects by using synchrotron-radiation-based micro-computed tomography and histopathological staining.METHODS:Thirty-two rabbits were randomly divided into the control group(n=12)and the experimental group(n=20)by the random number table method.Two holes with a size of 4 mm(the diameter of the cavity)×6 mm(hole depth)of penetrating periosteal bone defect were established on the lateral surface of one side of the femoral body.The control group was filled with normal saline.In the experimental group,Bio-oss bone powder was implanted in one of the bone defects,and Aobang repair material was implanted in the other bone defect.After 8 weeks,the effects of the two kinds of bone repair materials were evaluated by visual observation,imaging analysis and histopathological examination.RESULTS AND CONCLUSION:(1)General view:8 weeks after the operation,the wounds in the repaired area of bone injury in the experimental group healed well;no infection,suppuration or other symptoms were found,and the animal mobility was significantly better than that of the control group.(2)Imaging examination:synchrotron-radiation-based micro-computed tomography 8 weeks after the operation exhibited that after the implantation of Bio-oss bone powder,there was a large amount of new bone tissue around the bone powder,and the cavity of the bone defect was large,which was not completely filled by the new bone tissue.After the implantation of the Aobang repair material,a large amount of new bone tissue at the bone defect cavity was formed around the repair material.3D reconstruction showed that both bone filling materials could contact with local bone wound tissue,promote the surrounding osteogenesis to differentiate into new bone tis
作者
孙莲莲
刘永超
王志兴
Sun Lianlian;Liu Yongchao;Wang Zhixing(Department of Stomatology,Tianjin Fifth Central Hospital,Tianjin 300450,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第21期3343-3348,共6页
Chinese Journal of Tissue Engineering Research
基金
天津市滨海新区卫生计生委科技项目重点支持项目(2015BWKZ001),项目负责人:孙莲莲。
