摘要
为了提高化疗药物的安全性和有效性,以阳离子多糖壳聚糖为载体,抗癌药5-氟尿嘧啶和丹参素钠为药物模型,采用聚电解质复合法构建了新型控/缓释壳聚糖基复合纳米载药体系,考查了药物初始投加量、浓度、质量比对载药壳聚糖纳米颗粒粒径及其分布的影响。采用红外光谱仪、激光粒度仪、透射电镜对其结构、粒径及形貌进行表征,并研究了2种载药体系的体外药物释放行为以及壳聚糖载药体系的生物相容性。结果表明:对于载5-氟尿嘧啶体系,当质量浓度为0.8 g/L,质量比为5∶3时,粒径为382 nm;对于载丹参素钠体系,当质量比为10∶7时,粒径为425 nm。与游离药物相比,载药纳米颗粒控释效果更明显;初始投加量、pH及温度对药物释放速率都有影响;药物性质不同,释放速率及动力学行为不同。壳聚糖纳米颗粒生物相容性良好,符合生物医用材料的基本要求,可广泛应用于药物控释及基因载体等领域。
To improve the safety and effectiveness of chemotherapy drugs,a new controlled/sustained release chitosan-based composite nano drug delivery system was constructed by polyelectrolyte composite method with cationic polysaccharide chitosan as carrier,and anticancer drugs 5-fluorouracil and Salvianic acid A sodium as drug models.The effects of initial drug dosage,concentration and mass ratio of drugs on the particle size and distribution of drug-loaded chitosan nanoparticles were investigated.The structure,particle size and morphology were characterized by infrared spectroscopy,laser particle size analyzer and transmission electron microscope,and the in vitro drug release behavior of the two drug delivery systems was studied.L929 cells were used as a cell model to evaluate the biocompatibility of chitosan drug delivery system.The results show that 5-FU-loaded chitosan nanoparticles with particle size of 382 nm can be formed when the mass concentration was 0.8 g/L and the mass ratio was 5∶3.For Salvianic acid A sodium-loaded system,when the mass ratio was 10∶7,chitosan nanoparticles with particle size of 425 nm can be formed.The drug-loaded nanoparticles of the two systems has obvious controlled release effect compared with free drug.The initial dosage,pH and temperature have different effects on the drug release rate and release kinetics.The chitosan nanoparticles have good biocompatibility and meet the basic requirements of biomedical materials,which have a wide application prospect in the fields of drug control release and gene carrier.
作者
赵婧
崔潞
李映璐
王晓冉
张勇
ZHAO Jing;CUI Lu;LI Yinglu;WANG Xiaoran;ZHANG Yong(School of Environmental and Chemical Engineering,Xi’an Polytechnic University,Xi’an 710048,China)
出处
《纺织高校基础科学学报》
CAS
2022年第3期45-55,共11页
Basic Sciences Journal of Textile Universities
基金
陕西省重点研发计划项目(2020GY-321)
西安市科技计划项目(21XJZZ0017)。
关键词
壳聚糖
纳米载体体系
5-氟尿嘧啶
丹参素钠
药物控释
chitosan
drug delivery system
5-Fluorouracil
Salvianic acid A sodium
controlled drug release
