摘要
目的 探讨牙龈组织中MMP-13和TIMP-1的表达与游离龈移植术后应用CGF生长因子创面愈合的关系。方法 选取2019年6月至2021年12月沧州人民医院口腔科行游离龈移植术的患者82例作为研究对象,依据随机数字法分为观察组和对照组各41例,对照组或得游离龈瓣后,在供体处缝合碘仿纱条,观察组应用CGF薄膜覆盖硬腭供区创面,再用碘仿纱条缝合。比较不同的处理方式对创面愈合和牙龈组织中MMP-13、TIMP-1的影响,用Pearson相关性分析MMP-13和TIMP-1与创面愈合的关系。结果 随治疗时间的延长,观察组的创面面积愈合率、TIMP-1表达水平逐渐升高,观察组高于对照组(t=3.316、3.817、2.115、2.934、2.561、2.171、2.141、5.385,P<0.05),MMP-13表达水平逐渐下降,观察组低于对照组(t=2.561、2.196、5.000、5.327,P<0.05)。MMP-13高表达组创面愈合率均低于低表达组(t=2.218,P=0.032)。TIMP-1高表达组创面愈合率均高于低表达组(t=2.490,P=0.017)。Pearson相关性分析显示,观察组术后30 d创面面积愈合率与MMP-13表达水平呈负相关,与TIMP-1呈正相关。结论 行游离龈移植术后应用CGF生长因子治疗患者牙龈组织中MMP-13的表达水平逐渐下降,TIMP-1表达水平上升,两者与创面愈合率密切关联,CGF生长因子可能通过调控MMP-13和TIMP-1之间的关系以达到促进创面愈合的目的。
Objective To investigate the relationship between the expression of MMP-13 and TIMP-1 in gingival tissue and the wound healing after free gingival transplantation with CGF growth factor.Methods a total of 82 patients who underwent free gingival transplantation in the Department of Stomatology,Cangzhou People ’ s Hospital from June 2019 to December 2021 were selected as the research subjects.According to the random number method,41 cases were divided into the observation group and the control group respectively. After the free gingival flap was obtained in the control group,iodoform gauze strips were sutured at the donor site,while CGF film was applied to cover the hard palate donor site and then iodoform gauze strips were sutured in the observation group. The effects of different treatment methods on the wound healing and the levels of MMP-13 and TIMP-1 in gingival tissue were compared,and the relationship between MMP-13 and TIMP-1 and wound healing was analyzed by Pearson correlation. Results With the extension of treatment time,the wound healing rate and TIMP-1 expression level in the observation group were gradually increased,and the observation group was higher than the control group(t=3.316,3.817,2.115,2.934,2.561,2.171,2.141,5.385,P<0.05),while the expression level of MMP-13 was gradually decreased,and the observation group was lower than the control group(t=2.561,2.196,5.000,5.327,P<0.05). The wound healing rate in the high expression group of MMP-13 was lower than that in ther low expression group(t=2.218,P=0.032). The wound healing rate in the high expression group of TIMP-1 was higher than that in the low expression group(t=2.490,P=0.017). Pearson correlation analysis showed that the wound area healing rate in the observation group was negatively correlated with the expression level of MMP-13 at 30 days after operation,and positively correlated with TIMP-1. Conclusion After free gingival transplantation,the expression level of MMP-13 in the gingival tissue of patients treated with CGF growth factor gr
作者
于兰
于文凤
吕敏敏
YU Lan;YU Wenfeng;LV Minmin(Department of Stomatology,Cangzhou People's Hospital,Cangzhou,Hebei,China,061000)
出处
《分子诊断与治疗杂志》
2022年第10期1732-1735,1740,共5页
Journal of Molecular Diagnostics and Therapy
基金
沧州市重点研发计划指导项目(172302016)。
