摘要
目的评估液相色谱-电感耦合等离子体质谱法(liquid chromatography inductively coupled plasma mass spectrometry,LC-ICPMS)测定海产品中8种砷形态的不确定度。方法采用LC-ICP-MS测定海产品中砷胆碱(As C)、砷甜菜碱(As B)、亚砷酸(AsⅢ)、二甲基砷(DMA)、一甲基砷(MMA)、砷酸(AsⅤ)、卡巴胂(Carb)、洛克沙砷(ROX)含量,建立数学模型,并从样品称量、样品定容、标准溶液配制、标准曲线拟合、测量重复性等方面进行测量不确定度的计算。结果海产样品中As C、As B、AsⅢ、DMA、MMA、AsⅤ、Carb、ROX的含量分别为0.482 mg/kg、1.023 mg/kg、0.513 mg/kg、0.494 mg/kg、0.506 mg/kg、0.522 mg/kg、0.496 mg/kg、0.492 mg/kg,在95%的置信区间下,其扩展不确定度分别为0.023 mg/kg、0.039 mg/kg、0.016 mg/kg、0.021 mg/kg、0.021 mg/kg、0.018 mg/kg、0.013 mg/kg、0.013 mg/kg,k=2。结论测量过程中的不确定度主要来源于测量的重复性、标准溶液的配制,需要在测量过程中加以注意。
Objective To evaluate the uncertainty for determination of eight arsenic forms in seafood by liquid chromatography inductively coupled plasma mass spectrometry(LC-ICPMS).Methods The contents of As C,As B,AsⅢ,DMA,MMA,AsⅤ,Carb and ROX in seafood were determined by LC-ICPMS.The mathematical model was established,and the measurement uncertainty from the sample weighing,sample constant volume,standard solution preparation and standard curve fitting,measurement repeatability was calculated.Results When the contents of As C,As B,AsⅢ,DMA,MMA,AsⅤ,Carb and ROX in seafood were 0.482 mg/kg,1.023 mg/kg,0.513 mg/kg,0.494 mg/kg,0.506 mg/kg,0.522 mg/kg,0.496 mg/kg and 0.492 mg/kg,the expanded uncertainty was 0.023 mg/kg,0.039 mg/kg,0.016 mg/kg,0.021 mg/kg,0.021 mg/kg,0.018 mg/kg,0.013 mg/kg and 0.013 mg/kg,respectively(k=2)under the 95%confidence interval.Conclusion The uncertainty in the measurement process mainly comes from the preparation of standard solution and the repeatability of measurement,which should be paid attention to in the measurement process.
作者
葛淼华
吴小琼
高薇薇
周莹
张翔
GE Miao-hua;WU Xiao-qiong;GAO Wei-wei;ZHOU Ying;ZHANG Xiang(Jiaxing Municipal Center for Disease Control and Prevention,Jiaxing,Zhejiang 314050,China)
出处
《中国卫生检验杂志》
CAS
2022年第19期2329-2334,共6页
Chinese Journal of Health Laboratory Technology
