摘要
以4年生‘蒙原’欧李为试验材料,对不同生长阶段欧李叶片中多酚类物质(总酚、总黄酮、原花青素、花青素、黄酮醇)含量及其抗氧化活性(总抗氧化活性、铁离子还原力、DPPH·清除率、·OH清除率)进行了比较分析,为欧李叶片的进一步研究和开发利用提供理论依据。结果表明:(1)欧李叶片各酚类物质含量随着生长期均表现为先增加后降低的变化规律,并均于果实着色膨大期达到最大。(2)各酚类物质的抗氧化活性均表现出与各酚类物质类似的变化规律,除个别有差异外,基本也均于果实着色膨大期达到最高。(3)在果实着色膨大期,欧李叶片各酚类物质含量及其抗氧化活性均表现为总酚最高,总黄酮和原花青素次之,黄酮醇和花青素最低。研究发现,欧李叶片各酚类物质含量与其抗氧化活性存在着明显的协同变化关系,抗氧化活性随着各酚类物质含量的增加或降低而升高或降低;除原花青素和花青素外,着色膨大期是欧李叶片酚类物质含量积累高峰期,同时也是总抗氧化力最高的时期。
Weih 4-year-old‘Mengyuan’Cerasus humilis was used as the test material,this experiment conducted to explore the phenolic contents(total phenol,total flavonoids,proanthocyanidins,anthocyanin and flavonol)and antioxidant activities(total antioxidant,iron ion reduce power,DPPH·-clearance rate and·OH-clearance rate)of leaves in different growing stages of C.humilis,so as to provide theoretical basis for the further development and application of C.humilis leaves resources.The results show that:(1)the contents of phenolic substances in leaves of C.humilis increased firstly and then decreased,and reached the maximum at the pigmentation expansion stage.(2)The antioxidant activities of phenolic compounds were similar to that of phenolic compounds,and reached the maximum at the pigmentation expansion stage,except for individual differences.(3)During the pigmentation expansion stage,the contents of phenolic substances and their antioxidant activities in leaves of C.humilis were the highest,followed by total flavonoids and procyanidins,and flavonols and anthocyanins.In conclusion,the contents of phenolic compounds in leaves of C.humilis had obvious synergistic change relationship with its antioxidant activity,and the antioxidant activity increased or decreased with the increase or decrease of the contents of phenolic compounds.Apart from proanthocyanidin and anthocyanins,the accumulation peak of phenolic substances and total antioxidant capacity of C.humilis leaves reached the highest in the pigmentation expansion stage.
作者
邵郅胜
杨波
朱成
李东方
郭金丽
SHAO Zhisheng;YANG Bo;ZHU Cheng;LI Dongfang;GUO Jinli(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010018,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2022年第10期1720-1727,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
内蒙古科技成果转化专项(2019CG067)
新品种选育专项(YZGC2017019)。
关键词
欧李
叶片
酚类物质
抗氧化活性
Cerasus humilis
leaves
phenolics
antioxidant activity
