摘要
目的 探讨姜黄素对Ⅱ型胶原诱导RA大鼠炎症反应和破骨细胞生成的影响及其可能机制。方法30只SD大鼠随机分为对照组(Con)、RA模型组(RA)和姜黄素组(Cur),每组10只。观察各组大鼠踝关节关节肿胀情况;Alcian染色观察软骨蛋白多糖情况;TRAP染色观测破骨细胞;ELISA检测大鼠外周血IL-6及TNF-α含量;Western blot测定各组大鼠踝关节软骨组织TLR-4、NF-κB和MyD88蛋白的表达。结果 与对照组相比,模型组大鼠踝关节明显红肿、变形,大鼠出现跛行、活动障碍。姜黄素组大鼠踝关节红肿较模型组明显减轻,活动状况明显好转。RA组大鼠踝关节软骨组织及骨小梁组织中都出现了大量的破骨细胞,软骨层出现了明显退化,TLR-4、 NF-κB和MyD88蛋白表达明显升高(P<0.05),血清IL-6和TNF-α含量明显升高(P<0.05);经过姜黄素处理后,Cur组大鼠踝关节破骨细胞减少,软骨损伤减轻,TLR-4、NF-κB和MyD88表达降低,血清IL-6和TNF-α含量降低(P<0.05)。结论 姜黄素能够抑制RA大鼠的踝关节炎性反应和破骨细胞激活,其机制与下调TLR4/NF-κB信号通路有关。
Objective To investigate the effect of curcumin on inflammatory response and osteoclast formation induced by type II collagen in RA rats and its possible mechanism. Methods Thirty SD rats were randomly divided into control group(Con), RA model group(RA) and curcumin group(Cur), with 10 rats in each group. Ankle joint swelling of rats in each group was observed. Alcian staining was used to observe the proteoglycan of cartilage. Osteoclasts were observed by TRAP staining. The contents of IL-6 and TNF-α in peripheral blood were detected by ELISA. The expression of TLR-4,NF-κB and MyD88 protein in ankle cartilage of rats in each group was determined by Western blot. Results Compared with control group, the ankle joint of rats in model group was obviously red, swollen and deformed, and the rats showed claudication and mobility disorder. Compared with the model group, the ankle joint redness and swelling of rats in curcumin group were significantly reduced and their activity status was significantly improved. In RA group, a large number of osteoclasts were found in ankle cartilage and bone trabecular tissue, and the cartilage layer was degraded significantly, and the protein expression of TLR-4, NF-κB and MyD88 were significantly increased(P<0.05). The contents of IL-6 and TNF-α in peripheral blood were significantly increased(P<0.05). After curcumin treatment, osteoclasts were decreased,cartilage injury was relieved, the expression of TLR-4, NF-κB and MyD88 were decreased(P<0.05). Contents of IL-6 and TNF-α in peripheral blood were decreased. Conclusion Curcumin can inhibit ankle inflammatory response and osteoclast activation in RA rats, and the mechanism is related to down-regulation of TLR4/NF-κB signaling pathway.
作者
丁子阳
于其华
DING Zi-yang;YU Qi-hu(Graduate School of Anhui University of Traditional Chinese Medicine,Fuyang 230012;Department of Orthopaedics and Traumatology,Taihe County Hospital of Traditional Chinese Medicine,Anhui University of Chinese Medicine,Fuyang 236699,China)
出处
《解剖科学进展》
CAS
2022年第3期257-260,共4页
Progress of Anatomical Sciences
基金
安徽高校省级自然科学研究项目(KJ2018A352)。
