摘要
【目的】分析2017—2019年云南省德宏傣族景颇族自治州(简称“德宏州”)新报告人类免疫缺陷病毒(HIV)感染者基因亚型分布特征,探究HIV流行情况及亚型分布特征随时间的变化趋势。【方法】对德宏州2017—2019年新报告且血浆量>200μL的HIV感染者标本进行核酸抽提,使用反转录⁃聚合酶链反应(RT⁃PCR)方法对HIV的gag、env和pol基因进行逆转录扩增,并对扩增产物测序,根据上述3个基因测序结果进行亚型确定。【结果】2017—2019年德宏州共报告HIV感染者3287例,其中成功获得HIV的gag、env和pol基因亚型者1813例,主要优势亚型为C亚型(28.4%,515/1813)、BC重组亚型(22.0%,398/1813)和CRF01_AE亚型(18.1%,329/1813)。在中国籍与缅甸籍HIV感染者中均表现为B亚型、C亚型和CRF01_AE亚型所占构成比逐年下降,01/BC重组亚型、CRF07_BC亚型及CRF08_BC亚型的构成比逐年上升(χ^(2)=75.212,P<0.001)。中国籍与缅甸籍不同HIV基因亚型的HIV感染者性别、年龄、婚姻状态、民族、文化程度和传播途径方面的构成比差异均有统计学意义(均P<0.05)。【结论】德宏州HIV基因亚型分布随时间变化表现出更为复杂多样的特点,表现为BC重组亚型和独特重组型所占构成比明显增加。
[Objective]To determine the trend and influencing factors of HIV subtypes in newly reported HIV/AIDS cases in Dehong Dai and Jingpo Autonomous Prefecture(Dehong Prefecture)from 2017 to 2019.[Methods]RNA extraction was conducted among newly reported HIV/AIDS cases in Dehong Prefecture from 2017 to 2019 whose plasma volume was more than 200μL.The gag,env and pol genes were amplified by using RT-PCR and then sequenced to determine the subtypes.[Results]A total of 3287 HIV-infected cases were newly reported in Dehong from 2017 to 2019.The HIV gag,env and pol genotypes were determined in 1813 cases.The major subtypes were subtype C(28.4%,515/1813),recombination form BC(22.0%,398/1813)and CRF_01AE(18.1%,329/1813).Furthermore,the proportion of subtype B,subtype C and CRF01_AE decreased over years,whereas 01/BC,CRF07_BC and CRF08_BC increased over years in both Chinese and Burmese patients(χ^(2)=75.212,P<0.001).There were significant differences in gender,age,marital status,ethnicity,educational level and transmission route among Chinese and Burmese HIV-infected cases with diverse HIV genotypes(all P<0.05).[Conclusion]HIV subtypes in Dehong change over time,which demonstrates that the proportion of BC recombinant subtypes and unique recombinant subtypes increased significantly.
作者
段星
张志一
王继宝
叶润华
杨锦
周素娟
王译葵
杨涛
杨跃诚
唐仁海
何纳
丁盈盈
段松
DUAN Xing;ZHANG Zhiyi;WANG Jibao;YE Runhua;YANG Jin;ZHOU Sujuan;WANG Yikui;YANG Tao;YANG Yuecheng;TANG Renhai;HE Na;DING Yingying;DUAN Song(Department of STD and AIDS Prevention,Dehong Dai and Jingpo Autonomous Prefecture Center for Disease Control and Prevention,Mangshi,Yunnan 678400,China;Department of Epidemiology,School of Public Health,Key Laboratory of Public Health Safety,Ministry of Education,Fudan University,Shanghai 200032,China)
出处
《上海预防医学》
CAS
2022年第9期835-841,共7页
Shanghai Journal of Preventive Medicine
基金
国家科技重大专项(2018ZX10715006-001、2018ZX10721102-004)
国家重点地区艾滋病防治项目(卫办疾控发[2004] 49号)
