摘要
目的探讨GLI家族锌指蛋白3(GLI family zinc finger 3,GLI3)在胃癌的表达和作用,并分析其机制。方法用免疫组织化学法观察GLI3在胃癌组织中的表达,Western blot检测GLI3在胃癌细胞系中的表达,shRNA干扰法敲低胃癌细胞中GLI3的表达,CCK-8和EDU染色法评估细胞增殖,Transwell法检测细胞迁移与侵袭,免疫荧光染色观察β-连环蛋白(β-catenin)的表达和分布,Western blot法检测细胞中β-catenin以及上皮间质转化表型相关蛋白N-钙粘蛋白和E-钙粘蛋白的表达。结果GLI3表达在正常胃粘膜组织中呈阴性,在胃癌组织中呈阳性且随着分期的增加而增强。GLI3在胃癌细胞系中的表达高于正常胃黏膜细胞系GES-1。敲低GLI3抑制胃癌细胞增殖、迁移与侵袭,降低β-catenin和N-钙粘蛋白水平,增加E-钙粘蛋白水平。结论GLI3促进胃癌进展;敲低GLI3可抑制胃癌细胞增殖、迁移与侵袭,其作用可能与抑制上皮间质转化和β-catenin表达有关。
Objective To investigate the expression and function of GLI family zinc finger 3(GLI3)in gastric cancer,and to analyze its mechanism.Methods Immunohistochemistry was used to observe the expression of GLI3 in gastric cancer.The expression of GLI3 in gastric cancer cell lines was detected by Western blot.Knockdown of GLI3 in gastric cancer cells by shRNA interference,cell proliferation was assessed by CCK-8 and EDU staining,cell migration and invasion were detected by Transwell assay;the expression and distribution ofβ-catenin was observed by immunofluorescence assay;the expression ofβ-catenin,and EMT phenotype-related proteins N-cadherin and E-cadherin were detected by Western blot.Results GLI3 expression was negative in normal gastric mucosa but positive in gastric cancer and increased with increasing stage.The expression of GLI3 in gastric cancer cell line was higher than that in normal gastric mucosa cell line GES-1.GLI3 knockdown inhibited the proliferation,migration and invasion of gastric cancer cells,promoted the release ofβ-catenin and decreased the expression ofβ-catenin and N-cadherin while increased the expression of E-cadherin.Conclusion GLI3 promotes gastric cancer progression.Knockdown of GLI3 can inhibit the proliferation,migration and invasion of gastric cancer cells,which may be related to inhibition of epithelial-mesenchymal transformation andβ-catenin expression.
作者
袁博
常占国
马磊
Yuan Bo;Chang Zhanguo;Ma Lei(Department of Oncology Hematology,Nanyang First People’s Hospital,Nanyang 473000,China;Department of Oncology,Nanyang First People’s Hospital,Nanyang 473000,China)
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2022年第4期367-373,共7页
Chinese Journal of Histochemistry and Cytochemistry
基金
河南省医学科技攻关计划省部共建项目(SB201901101)。
关键词
胃癌
GLI3
增殖
迁移
侵袭
上皮-间质转化
Β-连环蛋白
Gastric cancer
GLI3
proliferation
migration
invasion
epithelial-mesenchymal transition
β-catenin
