摘要
从半滑舌鳎养殖尾水处理系统生物填料中分离纯化出7株细菌,用16S rDNA序列分析对分离的菌株进行鉴定,通过对7株菌株氨氮去除能力检测得到高效硝化菌株2株,并对其进行形态学观察和对养殖尾水脱氮效果的初步研究。结果表明,分离纯化得到的7株细菌分别为脱氮鲍曼氏菌(WY1)、麦氏交替单胞菌(WY3)、仙河盐单胞菌(WY4)、杜氏硫杆菌(WY5)、黄醇假交替单胞菌(WY6)、红色假交替单胞菌(WY8)和施氏假单胞菌(WY10)。经初步筛选,WY10菌株和WY4菌株的氨氮去除能力显著,去除率分别为53.99%和64.52%。革兰氏染色结果表明2株菌株均为革兰氏阴性菌,处理养殖尾水24 h后,WY4菌株氨氮、亚硝酸态氮、硝酸氮去除率分别为95.36%、99.65%、86.53%,WY10菌株氨氮、亚硝酸态氮、硝酸氮去除率分别为72.89%、94.40%、82.90%,2株菌的24 h脱氮效果显著。因此筛选出的2株硝化细菌具有调控海水养殖尾水的潜在应用价值。
Seven strains of bacteria were isolated and purified from the biological filler in the tail water treatment system of half-smooth tongue sole,and the isolated strains were identified by 16 S rDNA sequence analysis.Two strains of high-efficiency nitrifying strains were obtained by detecting the ammonia nitrogen removal ability of the seven strains,and morphological observation and preliminary research on denitrification effect of aquaculture tail water were carried out.The results showed that seven strains of bacteria were isolated and purified,which were Bowmanella denitrificans(WY1),Alteromonas macleodii(WY3),Halomonas xianhensis(WY4),Sulfitobacter dubius(WY5),Pseudoalteromonas luteoviolacea(WY6),Pseudoalteromonas rubra(WY8),Pseudomonas stutzeri(WY10).According to preliminary screening,strain WY10 and strain WY4 had significant ammonia nitrogen removal ability,with removal rates of 53.99% and 64.52%,respectively.The gram staining results showed that both strains were gram-negative bacteria.After 24 h treatment of tail water,the removal rates of ammonia nitrogen,nitrite nitrogen and nitrate nitrogen of WY4 strain were 95.36%,99.65% and 86.53%,respectively.The removal rates of ammonia nitrogen,nitrite nitrogen and nitric nitrogen of WY10 strain were 72.89%,94.40% and 82.90%,respectively.The denitrification effect of the two strains in 24 h was remarkable.Therefore,the two nitrifying bacteria screened in this study have potential application value in regulating the tail water of mariculture.
出处
《安徽农业科学》
CAS
2022年第19期110-113,129,共5页
Journal of Anhui Agricultural Sciences
基金
现代农业产业技术体系专项资金(CARS-47-Z01)
天津市海水养殖现代农业产业技术体系-海水鱼养殖岗位项目(ITTMRS2021001)
天津市农业发展服务中心青年科技创新项目(zxkj202118,zxkj202125)
天津市科技计划项目(21ZYCGSN00010)。
关键词
养殖尾水
硝化细菌
分离纯化
16S
rDNA
革兰氏染色
脱氮效果
Aquaculture tail water
Nitrifying bacteria
Isolation and purification
16S rDNA
Gram staining
Denitrification effect
