摘要
【目的】为了从分子生物学方面研究烟草K326雄性不育转录组相关基因的差异表达情况,明确烟草K326雄性不育的发生机制。【方法】以盛花期烟草K326雄性不育系和保持系不同大小花蕾为材料,利用Illumina HiSeq高通量测序技术,然后对测序原始数据进行相应的序列比对分析。【结果】在测序原始数据过滤后,总共得到了84 101 954~134 977 386个clean reads用于比对分析,碱基测序质量值Q30均大于94%,GC含量为41.84%~42.50%。小蕾、中蕾和大蕾中分别得到7881、19 931和10 984个差异表达基因,并且下调显著多于上调基因数。这些差异表达基因被注释到生物过程、分子功能和细胞组分这3大类35个分支中,在细胞组分层面中的分子功能的term数量最多。1257个差异基因被注释到KEGG数据库的249条代谢途径中,其中代谢途径、次生代谢物生物合成和苯丙烷生物合成3个通路所富集的差异基因数较多。对差异基因进行转录因子分析得知在小蕾、中蕾、大蕾时期较多的差异基因都属于ERF、MYB、C2H2、NAC、ERKY、bHLH等转录因子家族。【结论】本研究在对烟草雄性不育转录组育性相关基因的研究中发现MYB转录因子以及代谢途径和苯丙烷生物合成方面有较多差异表达基因,因此建议今后在挖掘烟草相关雄性不育基因及分子机制方面可在代谢途径和苯丙烷生物合成方面开展。
【Objective】The present paper aimed to study the differential expression of male sterility transcriptome-related genes from molecular biology, and to clarify the mechanism of male sterility in tobacco K326. 【Method】 The male sterile line and maintainer line of tobacco K326 at the flowering stage were used as materials, using Illumina HiSeq high-throughput sequencing technology. Then the corresponding sequence alignment analysis was performed on the sequencing raw data. 【Result】 After filtering the original sequencing data, a total of 84 101 954-134 977 386 clean reads were obtained for comparison and analysis. The base sequencing quality values Q30 were all greater than 94% and the GC content was between 41.84% and 42.50%. In small bud, medium buds and large buds, 7881, 19 931 and 10 984 differentially expressed genes were obtained respectively, of which the down-regulated were significantly more than the up-regulated. These differentially expressed genes were annotated to biological processes, molecular functions and cellular component 3 categories in 35 branches. The highest number of terms for molecular function was at the cellular component level. 1257 differential genes were annotated into the 249 metabolic pathways in the KEGG database. The most significantly enriched pathways in the KEGG metabolic pathways of differential genes were metabolic pathways, secondary metabolite biosynthesis and phenylpropane biosynthesis. Differential genes were analyzed by transcription factor analysis and it was found that the more differential genes belonged to ERF, MYB, C2 H2, NAC, ERKY, bHLH and other transcription factor families in small buds, medium buds and large buds. 【Conclusion】 In the study, we found that there were many differentially expressed genes in MYB transcription factors, metabolic pathways and phenylpropane biosynthesisin about male sterility. Therefore, we suggest that the exploration of tobacco-related male sterility genes and molecular mechanisms be carried out in the aspects of metaboli
作者
郑云
崔芳芳
郑九洲
杨祥飞
孟林峰
王建革
刘齐元
ZHENG Yun;CUI Fang-fang;ZHENG Jiu-zhou;YANG Xiang-fei;MENG Lin-feng;WANG Jian-ge;LIU Qi-yuan(The Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,College of Agriculture,Jiangxi Agricultural University,Nanchang 330045,China;College of Forestry,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《西南农业学报》
CSCD
北大核心
2022年第8期1733-1741,共9页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金(31960418)。
关键词
烟草
雄性不育
高通量测序
差异表达基因
Tobacco
Male sterility
High-throughput sequencing
Differentially expressed genes
