摘要
目的:建立藏荆芥中荆芥内酯的含量测定方法。方法:采用高效液相色谱法,色谱柱选择:Supersil ODS2 250 mm×4.6 mm,5μm,流动相为乙腈-水(体积比为40∶60),流速为1.0 mL/min,检测波长为216 nm,柱温为30℃。结果:荆芥内酯的线性关系方程为Y=12795176.3884X+607348.6713,R^(2)=0.9994,在0.01~5.0 mg/mL范围内的峰面积与进样浓度线性关系良好(R^(2)=0.9994)。精密度、重复性及稳定性实验的相对标准偏差(RSD)分别为1.46%、1.19%、1.83%,平均加样回收率为97.66%,RSD为1.56%。藏荆芥中荆芥内酯的含量为0.86%,不同药用部位中荆芥内酯含量差异明显。结论:建立的高效液相色谱法方法简单、准确、快速,重复性好,专属性强,可应用于藏荆芥中荆芥内酯的含量测定。
Objective: To establish a method for determination of Nepeta angustifolia C.Y.Wu. Methods: A high-performance liquid chromatography detection method was established. The column was Supersil ODS2 250 mm×4.6 mm, 5 μm and the mobile phase was acetonitrile-water(volume ratio 40∶60) at a flow rate of 1.0 mL/min. The detection wavelength was 216 nm, and the column temperature was 30 ℃. Results: The linear equation of nepetalactone was Y=12795176.3884X+607348.6713, and the peak area in the range of 0.01~5.0 mg/mL had a good linear relationship with the injection concentration(R^(2)=0.9994).The relative standard deviations(RSD) of precision, repeatability and stability experiments were 1.46 %, 1.19 % and 1.83 %, respectively. The average recovery rate was 97.66 % and RSD was 1.56 %. The content of nepetalactone in Nepeta angustifolia C.Y.Wu is 0.86 %, while which was obviously discrepant in different medicinal parts. Conclusion: The established HPLC method was simple, accurate, rapid, reproducible and specific, which could be applied to the determination of Nepeta angustifolia C.Y.Wu.
作者
杨阳
毛禹康
张琴
蔡鸿飞
李康强
张圳
许文东
Yang Yang;Mao Yukang;Zhang Qin;Cai Hongfei;Li Kangqiang;Zhang Zhen;Xu Wendong(Guangzhou Hanfang Pharmaceutical Co.,Ltd./National Engineering Research Center for Pharmaceutical Process Technology and New Drug Creation of Traditional Chinese Medicine,Guangzhou 510240,China)
出处
《广东化工》
CAS
2022年第20期212-215,共4页
Guangdong Chemical Industry
基金
国家科技部重点研发计划项目(2017YFC1703104)
广州市科技计划项目(202002020009)。
关键词
藏荆芥
荆芥内酯
含量测定
高效液相色谱
方法学
Nepeta angustifolia C.Y.Wu
nepetalactone
content determination
high performance liquid chromatography
methodology
