摘要
植物胞外囊泡与动物外泌体的结构和功能相似,在维持生物内环境稳态、预防多种疾病等方面具有重要作用,且其囊泡结构可作为递送载体,具有相当大的发展潜力。本文立意于对植物胞外囊泡的结构组成、提取方法、表征手段、抗炎活性及其在食品工业和药物递送方面的应用进行综述。本文介绍了植物胞外囊泡的概况,包括其结构和化学组成,归纳了现有的九种分离提取方法和七种表征手段,分析了这些方法的优点和不足,将有助于研究学者选择合适的提取和表征方法制备具有生物学功能的纳米级植物胞外囊泡。本文重点介绍了植物胞外囊泡的抗炎活性,特别是其在缓解炎症性肠病方面的研究成果;总结了植物胞外囊泡在食品工业发展中的应用,包括在饮品、食品加工以及作为壁材递送功能因子方面的潜在应用。本文还简述了植物胞外囊泡在药物递送方面的研究进展。本文将为探究植物胞外囊泡的生物学功能提供理论支持,拓展可食性植物来源胞外囊泡在食药研发方面的新应用。
The structure and function of plant extracellular vesicles(PEVs) are similar to those of animal-derived exosomes.Exosomes play important roles in maintaining the homeostasis of biological environment and preventing various diseases,more and more studies are focused on their bioactivities and applications. This article aims to review the structure and composition, extraction methods, characterization methods, anti-inflammatory activity of PEVs and their application in food and drug industry. In this paper, the overview of PEVs is introduced, including its structure and chemical composition;the advantages and disadvantages of different extraction and characterization methods are also summarized. It will help researchers to choose the appropriate method to prepare nano-sized PEVs with biological functions. This paper focuses on the anti-inflammatory activities of PEVs, especially research achievements in alleviating inflammatory bowel disease. The application of PEVs in food industry is summarized, including the potential application in beverage, food processing and as a delivery carrier of food functional factors. Advances in drug delivery by PEVs are also briefly reviewed. It will provide a theoretical support for exploring the biological functions of PEVs from edible plants and expand the new application of PEVs in food and drug industry.
作者
朱珍珠
江睿
廖柳月
张琳曼
谢丁宇
张婧艺
ZHU Zhenzhu;JIANG Rui;LIAO Liuyue;ZHANG Linman;XIE Dingyu;ZHANG Jingyi(College of Food Science and Engineering,Nanjing University of Finance and Economics,Collaborative Innovation Center for Modern Grain Circulation and Safety,Nanjing 210023,China)
出处
《食品工业科技》
CAS
北大核心
2022年第21期422-432,共11页
Science and Technology of Food Industry
基金
国家自然科学基金(31700714)
南京财经大学2020年青年学者支持计划(ZZZXW20001)
江苏省研究生科研创新计划(KYCX21_1518)
南京财经大学大学生创新训练项目(202110327205xj)
江苏高校优势学科建设工程资助项目。
关键词
植物胞外囊泡
提取方法
结构表征
抗炎活性
食品工业
递送体系
plant extracellular vesicles
extraction methods
structure characterization
anti-inflammatory activity
food industry
delivery system
