摘要
目的:探讨高压氧治疗对糖尿病大鼠末梢循环障碍与足部溃疡的疗效及其作用机制。方法:选取60只成年健康雄性SD大鼠,按照随机数字表法随机分为非糖尿病组、糖尿病假治疗组和高压氧治疗组,每组20只。注射2%链脲佐菌素建立糖尿病模型,以蒸气持续烫伤大鼠足底(面积约为37 mm 2)造成烫伤足溃疡模型。采用红外测温仪和足底测试仪分别测定大鼠双后足足底温度和热痛阈值,比较治疗前和治疗1、2、4周后3组大鼠的足底温度、足底热痛阈值。检测其创面组织中环氧化酶2(COX-2)、血管内皮生长因子(VEGF)、血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、超敏C反应蛋白(hs-CRP)水平和丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)水平。结果:治疗前糖尿病假治疗组和高压氧治疗组大鼠的足底温度低于非糖尿病组(P<0.05);3组大鼠的足底温度治疗前后比较,差异无统计学意义(P>0.05);治疗1周后,糖尿病假治疗组和高压氧治疗组大鼠的足底温度低于非糖尿病组(P<0.05);治疗2周和4周后,糖尿病假治疗组大鼠的足底温度低于非糖尿病组和高压氧治疗组(P<0.05)。治疗前,糖尿病假治疗组和高压氧治疗组大鼠的足底热痛阈值高于非糖尿病组(P<0.05);治疗1周后,高压氧治疗组大鼠的足底热痛阈值低于治疗前,糖尿病假治疗组和高压氧治疗组高于非糖尿病组(P<0.05);治疗2周后,非糖尿病组和糖尿病假治疗组大鼠的足底热痛阈值较治疗前降低,高压氧治疗组高于非糖尿病组,低于糖尿病假治疗组(P<0.05);治疗4周后,非糖尿病组和高压氧治疗组大鼠的足底热痛阈值较治疗前降低,糖尿病假治疗组高于非糖尿病组和高压氧治疗组(P<0.05)。治疗前3组大鼠的创面面积比较差异无统计学意义(P>0.05);治疗1周后,3组大鼠的创面面积较治疗前显著增大,非糖尿病组和高压氧治疗组大�
Objective To study the efficacy and mechanism of hyperbaric oxygen therapy on peripheral circulation disorder and foot ulcer in diabetic rats.Methods A total of sixty adult healthy male SD rats were selected and randomly divided into non-diabetic group,diabetic sham treatment group,and hyperbaric oxygen treatment group,with 20 rats in each group.The diabetic model was established by injecting 2%streptozotocin,and the model of foot ulcer model was established by continuous scalding the rat’s left posterior sole with steam(with an area of about 37 mm2).An infrared thermometer was used to measure the temperature of the rats’two posterior soles,and a plantar tester was used to measure the rats’plantar thermal pain thresholds.The plantar temperatures and plantar thermal pain thresholds of the rats in the three groups were compared before treatment and after 1 week,2 weeks,and 4 weeks of treatment.The levels of cyclooxygenase 2(COX-2),vascular endothelial growth factor(VEGF),serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),hypersensitive C-reactive protein(hs-CRP),malondialdehyde(MDA),superoxide dismutase(SOD),and catalase(CAT)in wound tissue were detected.Results Before treatment,the plantar temperatures of the rats in the diabetic sham treatment group and the hyperbaric oxygen treatment group were lower than that in the non-diabetic group(P<0.05);there was no significant difference in the plantar temperature within each group before and after treatment(P>0.05).After 1 week of treatment,the plantar temperatures of the rats in the diabetic sham treatment group and the hyperbaric oxygen treatment group were lower than that in the non-diabetic group(P<0.05);after 2 and 4 weeks of treatment,the plantar temperature of the rats in the diabetic sham treatment group was lower than those in the non-diabetic group and the hyperbaric oxygen treatment group(P<0.05).Before treatment,the plantar thermal pain thresholds of the rats in the diabetic sham treatment group and the hyperbaric oxygen
作者
孙之中
刘朝红
陈其华
易倩
刘莹莹
赵姣
林雅思
何欢
Sun Zhizhong;Liu Zhaohong;Chen Qihua;Yi Qian;Liu Yingying;Zhao Jiao;Lin Yasi;He Huan(The First Hospital of Hunan University of Chinese Medicine,Changsha 410000,China;Hunan University of Chinese Medicine,Changsha 410000,China)
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2022年第4期505-510,共6页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
湖南省临床医疗技术创新引导项目(2020SK51407)。
关键词
高压氧
糖尿病
末梢循环障碍
足部溃疡
大鼠
Hyperbaric oxygen
Diabetes
Peripheral circulation disorder
Foot ulcer
Rat
