摘要
为探究西藏牦牛ELOVL6基因的结构与功能,试验采用PCR技术克隆西藏牦牛ELOVL6基因编码区序列,利用在线软件预测和分析其编码氨基酸序列的生物信息学特征,并利用实时荧光定量PCR技术测定ELOVL6基因在西藏牦牛不同组织中的表达水平。结果表明:西藏牦牛ELOVL6基因编码区序列长度为795 bp,共编码264个氨基酸;与牛ELOVL6基因(GenBank登录号为NM_001102155.1)编码的氨基酸序列比较,其第18位核苷酸位点发生了碱基突变(A→C)。西藏牦牛与普通牛亲缘关系较近,与斑马鱼亲缘关系较远。ELOVL6基因编码蛋白是一种主要在内质网发挥生物学功能的疏水性蛋白,共有20个磷酸化位点,有跨膜结构,无信号肽,二级结构以α-螺旋为主。ELOVL6基因在西藏牦牛的不同组织中均有表达,其中,在脂肪组织中的相对表达量最高,极显著高于其他组织(P<0.01)。说明ELOVL6基因可能是调控牦牛脂肪酸代谢的关键基因。
In order to explore the structure and function of Tibet yak ELOVL6 gene,the coding sequence of Tibet yak ELOVL6 gene was cloned by PCR,and the bioinformatic characteristics of its coding amino acid sequence were predicted and analyzed by online software.The expression levels of ELOVL6 gene in different tissues of Tibet yak were determined by real-time fluorescence quantitative PCR.The results showed that the coding region of Tibet yak ELOVL6 gene was 795 bp,encoding 264 amino acids.Compared with the amino acid sequence encoded by the common bovine ELOVL6 gene(GenBank accession no.NM_001102155.1),there was a base mutation(A→C)at the 18 th nucleotide site.Tibetan yak was closely related to Bos taurus,and it had a further relation with Danio rerio.The protein encoded by ELOVL6 gene was a hydrophobic protein that mainly performed biological functions in the endoplasmic reticulum.It had a total of 20 phosphorylation sites and a transmembrane structure with no signal peptide and a primaryα-helix secondary structure.ELOVL6 gene was expressed in different tissues of Tibet yaks,and the relative expression level of ELOVL6 gene in adipose tissue was the highest and was significantly higher than that in other tissues(P<0.01).These results indicated that ELOVL6 gene might be a key gene regulating fatty acid metabolism in Tibet yaks.
作者
任稳稳
郑青波
黄纯
王兴东
阎萍
梁春年
REN Wenwen;ZHENG Qingbo;HUANG Chun;WANG Xingdong;YAN Ping;LIANG Chunnian(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China;Key Laboratory of Yak Breeding Engineering of Gansu Province,Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau,Ministry of Agriculture and Rural Affaris,Lanzhou 730050,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2022年第18期130-135,140,145,共8页
Heilongjiang Animal Science And veterinary Medicine
基金
西藏区域科技协同创新专项(QYXTZX-NQ2022-04)
财政部和农业农村部-现代农业(肉牛牦牛)产业技术体系建设专项(CARS-37)
甘肃省科技计划资助项目(20JR5RA580)
中国农业科学院创新工程项目(CAAS-ASTIP-2014-LIHPS-01)。
